FastQCFastQC Report
Tue 10 May 2022
EGAF00003667002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003667002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22711
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.3302364492976972No Hit
GTGTAATCTCCTGCCCCTGCTCCGGGTACCCCAGGGCTGCCGCCCATCAG710.31262383866848664No Hit
GTCCACGAGCAGTCCGTTGCCGCCGTTCATCAGCTTCGCGATGCGAACGA630.2773986174100656No Hit
GTACATGGGAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGAC570.25097970146624987No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG560.2465765488089472No Hit
GTCCACCACCTTGACGATGACCATGTCGGTCTCCTTGTCGACCTCGAACT480.21135132755052619No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG470.20694817489322354No Hit
ATCATAGGTTGCACCATGCGTCTCCGGGCATTAATGAACCAGTTGTTGAC450.1981418695786183No Hit
GGGTATTGTTGATCTTGAGAAGATTTTGTAGAGTGGCAGCAAATCCTGGT440.19373871692131567No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG400.17612610629210515No Hit
GAATAATATATGGATTCGAGACCATGAGGATAGCGGGTCTGTACATTTGG380.16731980097749988No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA370.16291664832019725No Hit
GTACATGGGCACCAGGGGCTGGCTCTCGGCGTCCTGGAGCTTCGAGAGCA360.15851349566289463No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG350.154110343005592No Hit
GTACATGGGGAGGGGGTTGGCGAAGCACACGAAGTGCGCGAAGCCTGGGG340.1497071903482894No Hit
GCCCTGGAAGGAGCAGGTCGCGGGGTCGGCCGTCACCGTCACGCCGGCGG330.14530403769098676No Hit
CACACACAGTGGCCAAAAAGAAAGCCTCACTGTGTAATCTCCTGCCCCTG330.14530403769098676No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC330.14530403769098676No Hit
CTGCAACCTCTGCCTCCCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCC330.14530403769098676No Hit
GTACATGGGCGAGGCCGCGTTCAACGACTGGGGCTGGCGCGTGCCGTTCC320.14090088503368411No Hit
CACACACACAGTGGCCAAAAAGAAAGCCTCACTGTGTAATCTCCTGCCCC320.14090088503368411No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT310.1364977323763815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.13209457971907887No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT300.13209457971907887No Hit
TATCAGCACTGTCAGTTGGGGTCTGTCCCCAGGACTCCATCCATGTTGCC290.12769142706177622No Hit
TCTTTATTGGGCATCAGCGCTGGTGTGGAGGAGGCTCCTGGTTCCACCCA290.12769142706177622No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA290.12769142706177622No Hit
GGCAGAGGGTATTGTTGATCTTGAGAAGATTTTGTAGAGTGGCAGCAAAT280.1232882744044736No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG280.1232882744044736No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT270.11888512174717097No Hit
GTCCAGGTTGGAGTGCAGGGGCGCAATCTCGGCTCACTGCAACCTCTGCC270.11888512174717097No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA270.11888512174717097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.11888512174717097No Hit
ACGCAGAGTACATGGGCACCAGGGGCTGGCTCTCGGCGTCCTGGAGCTTC260.11448196908986834No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA260.11448196908986834No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG260.11448196908986834No Hit
GAACTGGAAGATTGTCATTACTGCCGCAGCATGGAGACCCAGGATGGGAG260.11448196908986834No Hit
ATCCTGGTTGGAGTGAACTGAGGAGAAAATGGGAGGGGAGAAATGAGTCT260.11448196908986834No Hit
CCAGTGGACGCAGCTGCTCGTCGGCGGCGCCGCGGGCGCGGCCTCTGCCG250.11007881643256572No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG250.11007881643256572No Hit
TCCTATGCCTTCTTGAATTCGTATCGATGTGGTTGCTGGATTCGAGCCCG250.11007881643256572No Hit
GTCCCCAGGACTCCATCCATGTTGCCACCATCTCTCCCTGGGCATCTGCT250.11007881643256572No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA240.10567566377526309No Hit
GGTCCAGGTCGAGCAGGCGGTGCGCCAGGTGAACGAGTCGCTCGCGCTGC240.10567566377526309No Hit
CCCCAGGGCTGCCGCCCATCAGGGTAAAGTCCAAGTGGCCAACATGATGC240.10567566377526309No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG230.10127251111796046No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC230.10127251111796046No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC230.10127251111796046No Hit
CCTAAACGTTCTTCCTTTTCTATCTAGTCACCTTCCACCCGTTCCACACA230.10127251111796046No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA230.10127251111796046No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT230.10127251111796046No Hit
TCCCCAAGGTGGCCACCAACATCATGCGAGCCTGGTTGTTCCAGCACCTC230.10127251111796046No Hit
ATATTATTCTGGTCTGGGAGGCTGAGGCAGGAGGCTGGGTAGTCCTCGAA230.10127251111796046No Hit
CTCCCTGGGCATCTGCTGTGGCCTCCTAAACGTTCTTCCTTTTCTATCTA230.10127251111796046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)