FastQCFastQC Report
Tue 10 May 2022
EGAF00003667609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003667609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73747
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4440.6020583888158162No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3310.4488318168874666No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC3240.43933990535208217No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT3180.4312039811788954No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA2960.4013722592105442No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT2750.3728965246043907No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG2450.33221690373845714No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT2440.33086091637625936No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA2350.31865703011647933No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT2150.2915372828725236No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA2020.27390944716395244No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC2010.27255345980175466No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC1920.2603495735419746No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT1860.2522136493687879No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG1810.24543371255779894No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT1750.23729778838461227No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG1710.23187383893582114No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA1680.22780587684922776No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1660.2250939021248322No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG1550.21017804114065655No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT1530.20746606641626103No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT1490.20204211696746985No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG1480.2006861296052721No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1460.1979741548808765No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA1450.19661816751867872No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT1450.19661816751867872No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG1410.19119421806988757No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1400.18983823070768982No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC1390.18848224334549202No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG1370.18577026862109644No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA1360.18441428125889867No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG1350.1830582938967009No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA1340.1817023065345031No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC1320.1789903318101075No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG1260.17085440763692084No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT1260.17085440763692084No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT1250.16949842027472303No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT1250.16949842027472303No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA1240.16814243291252526No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT1230.16678644555032746No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT1180.16000650873933853No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC1180.16000650873933853No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA1140.1545825592905474No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG1120.15187058456615185No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT1110.15051459720395405No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA1110.15051459720395405No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG1100.14915860984175627No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG1100.14915860984175627No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG1070.14509064775516292No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA1040.14102268566856957No Hit
GGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAG1030.1396666983063718No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC1020.138310710944174No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA1020.138310710944174No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT1010.13695472358197622No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC1010.13695472358197622No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG1010.13695472358197622No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT990.13424274885758064No Hit
ATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAA990.13424274885758064No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT980.13288676149538287No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC980.13288676149538287No Hit
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT980.13288676149538287No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC970.1315307741331851No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA960.1301747867709873No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA960.1301747867709873No Hit
GCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGC960.1301747867709873No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC940.12746281204659174No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG940.12746281204659174No Hit
CACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTG930.12610682468439394No Hit
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCC910.12339484995999837No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC910.12339484995999837No Hit
CGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAAT900.1220388625978006No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC880.11932688787340504No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA870.11797090051120723No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT860.11661491314900944No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA860.11661491314900944No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA860.11661491314900944No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC850.11525892578681167No Hit
ATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGC850.11525892578681167No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT840.11390293842461388No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT830.1125469510624161No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG830.1125469510624161No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA830.1125469510624161No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG820.1111909637002183No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC820.1111909637002183No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT820.1111909637002183No Hit
CCCTTGTCCCGTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAA820.1111909637002183No Hit
AGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTC800.10847898897582274No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC800.10847898897582274No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG800.10847898897582274No Hit
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC790.10712300161362495No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC790.10712300161362495No Hit
GTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCAT790.10712300161362495No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA780.10576701425142718No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC780.10576701425142718No Hit
CTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACG770.10441102688922939No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG770.10441102688922939No Hit
AACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTG770.10441102688922939No Hit
GTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTC760.10305503952703161No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG760.10305503952703161No Hit
AAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAG760.10305503952703161No Hit
GTTATAATCGACCCGGGCTGCGACAATCGGCCGTATGCGGCGGTTGTTCT750.10169905216483383No Hit
AATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTG750.10169905216483383No Hit
GAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGC750.10169905216483383No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG750.10169905216483383No Hit
GCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTT750.10169905216483383No Hit
ACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGA750.10169905216483383No Hit
GTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACC740.10034306480263605No Hit
GAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATAT740.10034306480263605No Hit
CTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCG740.10034306480263605No Hit
GTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGG740.10034306480263605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)