FastQCFastQC Report
Tue 10 May 2022
EGAF00003667769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003667769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481424
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG16320.33899431685998205No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG14390.29890491541759445No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA10930.22703479676958355No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG9250.19213832297517366No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC8890.18466050716208582No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT7420.15412609259197715No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT7070.1468559938848084No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC6830.14187078334274983No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC6790.14103991491907342No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG6750.14020904649539698No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC6610.13730100701252948No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG6430.13356209910598557No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG6290.1306540596231181No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT6260.13003090830536077No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6240.12961547409352256Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC6080.12629200039881683No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC5960.12379939512778756No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC5920.12296852670411114No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG5840.12130678985675829No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT5750.11943733590348633No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC5560.1154907108910233No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC5550.1152829937851042No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA5240.1088437635016119No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC5160.10718202665425904No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA5050.10489713848914886No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT5040.10468942138322976No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT4970.103235401641796No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA4860.10095051347668582No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT4820.10011964505300941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)