FastQCFastQC Report
Tue 10 May 2022
EGAF00003668070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003668070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8672
Sequences flagged as poor quality0
Sequence length146
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA2412.779059040590406No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1772.0410516605166054No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA210.2421586715867159No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT200.23062730627306272No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG180.20756457564575648No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.1960332103321033No Hit
GTACATGGGATGCCTCCTCCGGACACAAGTACTCCCTGAAAGAGGCCCTT150.17297047970479704No Hit
GTACATGGGAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAAC150.17297047970479704No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG140.16143911439114392No Hit
GTACATGGGATGTGGAGAAGAGCCAATTTAACAAACTAGGAAGATGAAAA140.16143911439114392No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT140.16143911439114392No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA130.14990774907749077No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT130.14990774907749077No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT130.14990774907749077No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT130.14990774907749077No Hit
GAATTATATAATTCTAACGCTTAAATCATGTGAAAGGGTTGCTGCTGTCA120.13837638376383762No Hit
GTACATGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCT120.13837638376383762No Hit
ACATAATACAAAAACATGAAACCAACCATCTTCAGCCCACACTTTCTGGC120.13837638376383762No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG110.1268450184501845No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT110.1268450184501845No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT110.1268450184501845No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT110.1268450184501845No Hit
GTATCAACGCAGAGTACATGGGTATTTTTGCAAAGCGGGCGAGAACGAGA110.1268450184501845No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA110.1268450184501845No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG100.11531365313653136No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG100.11531365313653136No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG100.11531365313653136No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG100.11531365313653136No Hit
CGCTTAAATCATGTGAAAGGGTTGCTGCTGTCAGCCTTGCCCACTGTGAC100.11531365313653136No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA90.10378228782287824No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC90.10378228782287824No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG90.10378228782287824No Hit
GGCTTAACCCTAGAGACACAGTAGACCAGTTTCAGCTTACATTTAAGATG90.10378228782287824No Hit
TCTTTATTCCTCTCCTGCCTCAGAGGTCAGGAAGGAGGTCTGGCAGGACC90.10378228782287824No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA90.10378228782287824No Hit
TATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCCA90.10378228782287824No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC90.10378228782287824No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT90.10378228782287824No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC90.10378228782287824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)