FastQCFastQC Report
Tue 10 May 2022
EGAF00003668090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003668090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331681
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG19270.5809799174508037No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA15360.4630955647142887No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG13590.4097310367491656No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC12640.3810890584628001No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT11840.35696949780059756No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC11560.34852765156882665No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG11520.3473216735357166No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG10740.32380510189006906No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG10100.30450945336030705No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT9450.28491231032226744No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC9410.28370633228915737No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG8500.25627033203590194No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC7610.22943732079920165No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA7520.22672387022470386No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG7000.21104615579427222No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA6870.20712672718666428No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT6360.19175050726451018No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC5790.17456532029269087No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG5760.1736608367678583No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA5700.1718518697181931No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA5500.16582197955264244No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC5410.16310852897814468No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG5370.16190255094503453No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG5150.15526967176292883No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA5100.15376219922154116No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA4980.1501442651222108No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA4890.147430814547713No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT4750.14320989143182758No Hit
CTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGC4710.14200391339871743No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT4660.1404964408573298No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAG4650.14019494634905225No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA4640.13989345184077473No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC4510.13597402323316682No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA4400.13265758364211397No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA4390.13235608913383642No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA4370.13175310011728136No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC4340.13084861659244876No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC4320.1302456275758937No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4250.12813516601795097No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT4180.12602470446000827No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA4150.12512022093517566No Hit
GTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT4130.1245172319186206No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT4120.12421573741034307No Hit
CAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACA4070.1227082648689554No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA3920.11818584724479243No Hit
GATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCA3910.11788435273651492No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG3850.11607538568684972No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC3850.11607538568684972No Hit
GTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGC3850.11607538568684972No Hit
CCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACAC3840.11577389117857217No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT3810.11486940765373958No Hit
GGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT3790.11426641863718454No Hit
TGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATG3770.11366342962062946No Hit
AGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAAT3750.11306044060407439No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC3660.1103469900295766No Hit
GCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAA3660.1103469900295766No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG3630.109442506504744No Hit
GCGGTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGA3620.10914101199646649No Hit
CGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACC3560.10733204494680129No Hit
TAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTG3550.10703055043852377No Hit
TCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCC3490.10522158338885858No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG3480.10492008888058103No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA3470.1046185943723035No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT3420.10311112183091584No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC3400.10250813281436079No Hit
CTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAG3390.10220663830608324No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG3390.10220663830608324No Hit
GTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT3380.10190514379780573No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC3370.1016036492895282No Hit
TATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACT3350.10100066027297312No Hit
TTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTG3350.10100066027297312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)