FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003668283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003668283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAGCGCCGACTCGGCCTGTGCGAGCACGCCGCGCGCGGCGTCCACCT412.5No Hit
GTGCAGGAATGGTAATGCCCGGTGTCACAGGTATGATTGCTGATATTTCT39.375No Hit
CTGGCCGACTGGTGGCAGGAATTCAGCCTGGCCGATGAGGCCACGCGCGA26.25No Hit
GATTACAAGCGTGAGCCACTGCGCCTGGCCAGAAGACTGACGTTTTATAA26.25No Hit
GTTAACGGACCTGGAGGCCATCAAGAGCAAGGCACTGGCGGACATCGACG26.25No Hit
TCTCTCGGGTTTATATGGGCACAGGATGGGGGGTGTGGCTGTCTCTTATA26.25No Hit
GTCATTGTATCTTTACCAACTGAAGCTTCGCTCAATTTTGTATAAAATGC26.25No Hit
GTCCGAATACGGTCGGCGTCGAGGTATTCCGTTATTGACTGACGGCACAG13.125No Hit
GGTCCAAAGTTCCCAGAAAGCCATTTAGAACAGCCAGACACACACAAATA13.125No Hit
GTCGACTTGCGTCGTGGTTGTCGTGACGGTCTCGTTGGGTTGCTCGCCGA13.125No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA13.125No Hit
AGCATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA13.125Clontech Universal Primer Mix Long (95% over 22bp)
ACACCAATGGTCGTCAGGTCGTCCCCGGACCGCCTCGAGGTTTTCTCATC13.125No Hit
GCTGAAATGCCGAAGCAAAAGCTGTTGGCGTTGGCAGCAGCCAGATTGTT13.125No Hit
TCGCATCGGACTGGCGCACCTGCAACGTCGAGGCGCAGTCGCGCGATCCG13.125No Hit
TCTTTAGACAGAACAGTTTTGAAACACTCTTTTTGTGGAATCTGCAAGTG13.125No Hit
GTTCGCGGCGTCCAGACGGTCAAACAGACCTGGGCCGCCCTCGATGGCAT13.125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT13.125No Hit
CAGGTAGACCTCGGCCTGGTCGGCGGAGAACAGCGACACCGCGTCGCCCG13.125No Hit
ATGGTAGGTATTACTTATTATTAATTTTGAGATTTTTCTGCACGTAAACT13.125No Hit
GTCATATATAGACAGGTCTTTCCCCCGTTTCCTCTACTTTTTCTTGTAGC13.125No Hit
CTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTG13.125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)