FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003668285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003668285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAAATAGTGCTTCATTGAGTTATGCATATCTTCCAAGTGCTGACAGAT29.090909090909092No Hit
AACCTGCTCTATGAAAGGGACTGTTCAACACTGTGACTTCAATTGAAACA29.090909090909092No Hit
TCCCATCTCAGCCTCCCAAGTAGCTGGGACTATAAATTAGCTGGCACTAC14.545454545454546No Hit
GTTTTCCTTCTGCTCTGTGCCTCTTGCTTCTTGCATCTTCTGTGGGTGCC14.545454545454546No Hit
GTCAGTGCCCTGCGCCGAGGTGGTGCAGCAGGTCGTGGACGACGTCGTCG14.545454545454546No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATGGGTTTATACCACA14.545454545454546No Hit
GCTTTGGGTAGTCTGGTCATTTTAACCATATTAATTCTTCTGATCCTTAT14.545454545454546No Hit
CACTGCAACCTCCGCCTCCTGAGTTCAAGCAATTCTAAATTATCATGCCT14.545454545454546No Hit
TCGCTGCGCTTCTGGGCCCGCGGCGGGCGTGGGGCTGCCCGGGCCGGTCG14.545454545454546No Hit
CTCCAAGGCCGAGGATCGTACTGATTCCAAGGGAAATCTGGGAGCAAGGC14.545454545454546No Hit
CCCAAGCACCTTCCAGATCACAGACATGACCCGCAGGAGCTGCCAGAACC14.545454545454546No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT14.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
ATCTAGCCCTTGCAGTTCTTTTTTGTTTTCTGGGAGGCTTTTAATCTGTC14.545454545454546No Hit
ATGGAAGGCAGCGCCACGTGCGCGTCCTTCCCCAGGAAAGCGGGCGAATA14.545454545454546No Hit
CCCTTGTAGGTCGACAGCAGGTAGCTGAAGAACAGGATGATGGCCGCGAA14.545454545454546No Hit
CAGTGACGTTGAGCACTTTTTCATATGCTTGTTGGCTGGACGAACGTCTT14.545454545454546No Hit
GACCTGGCCATGGATAACCGTCACCGGCGCCGCCGTCATAGGATCGACGC14.545454545454546No Hit
GAAGTAGCTCATGCAGATGGGACAGGTGAGTGCCCTCTGGAAGACTTGCA14.545454545454546No Hit
CTTATCAAGGAGTTGTGTGTGTGTTTTGCATACAACTTTACAATTTCATA14.545454545454546No Hit
AGGAGGAAGAAATGGTTTCTTGGGCCAGGCCCCTGCTGTGTGCATCCTAG14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)