FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003668286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003668286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGAAACACTCTTTTTGTAAAATCTGCAAGAGGATATTTGGATAGCT29.090909090909092No Hit
TTTTAAAACTCATATCTACCTCTATGAATTTGGCAGCTTCTTGATTCTTC29.090909090909092No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14.545454545454546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGGAAATCCGCAAGCCTGTCTTCAACCTGAAAAACGCACGGGCCAAGCT14.545454545454546No Hit
TCCACTGTCCATTTGTGTTGGGTAAGAAACCTATGTCTTCATTCACTGCA14.545454545454546No Hit
GTCAAGGTGAACATCACCAGTCATGAGTAAAATGGAAATCCTGCATCAAC14.545454545454546No Hit
AAGCAAAATGGTGCCCCCTCCCGTAGACAGATGCAGCTCTGCTCCAGGGA14.545454545454546No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT14.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
GCTGACGCTGGCGCTGCTTGCGGGGCAGTCGGTGGGGCCGTTCCCGGTGG14.545454545454546No Hit
CCAAAATGACATGCTGCTCTTTCTTCTTCAGAAACATGAATTCTGGAATC14.545454545454546No Hit
ATCGCCCAGAGCACATGTGTTAGGTAAAATGGTCCAAGGACAGAGCTTTG14.545454545454546No Hit
ATTAAAAGCCTCCCAGAAAACAAAAAAGAACTGCAAGGGCTAGATCTGTC14.545454545454546No Hit
GTCAGGTACAGCTCTGGCTGTGCAAGCCCCAAGCCTCTGCAGCCACGTGG14.545454545454546No Hit
GCACCAAGTAGCACCCCTGAGGCTTGGGCTCCAGCAGCCTCTCTGCCTCC14.545454545454546No Hit
TAACCAAAGAGACCAACTTCCAACTATGTCCTATCCCCATTCCAAATGAA14.545454545454546No Hit
ACCCGCGACCGGGCCGGGAAGGGCGTCCCCAGCCTCCCGCGCCACGCGCG14.545454545454546No Hit
GGCGGAGCGCTTCGCGCTGCTGGCCGACCCGGGCCGGCTGCGCCTGCTCT14.545454545454546No Hit
ATGCAGAAGACAGAAACAGGTGGAAAGATATCCCACGCATAAGGATCAGA14.545454545454546No Hit
GCAACGAACATGACGCACAGGGAGATTTTCCCGTGGCGATCTTGGGCGGG14.545454545454546No Hit
GACTCTGTCTCAAAAAAAATAAATAAATAAAAATAAAACAGGACTGTCCA14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)