FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003668369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003668369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTACTGGGCTTAACCTGCTTCGACGGGGCAGCTTGCTTACTCGGCTG35.88235294117647No Hit
CCGTGGTCGGCGTGGCCGACGCGCTCAAGGGGCAGGTGGCCATGGCCTTC35.88235294117647No Hit
AGATGATTCCGGTGCTCATCGGGACGACGTTCATCATTTTCTGTTTGGTC23.9215686274509802No Hit
GAACTGGGACCGGGTGCCTGAGGGTGGAGGTTGCCCGGGGCCACCTGGGC23.9215686274509802No Hit
ACCCAGGTTTGGGCCCTCTAGATGGAGGTACTACCCTACTTCCTGCTTGA23.9215686274509802No Hit
ACAGTATGTTGTTTCCTCAAAAAACTGAAAATAGAAGTACAATATGATCC23.9215686274509802No Hit
GTGCGTCACCACCGACGAGCACATCTCCCACCTTGATCTGGTGCGTCTTA23.9215686274509802No Hit
GAGTAATCGGCCATGAGGACGACAATTCCGTGATTCGCACATCGGGGACC23.9215686274509802No Hit
TAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAA23.9215686274509802No Hit
GTCACAGCACGGTGACGTCGAGTTCCTCGCGCAGAACTCCCGACGATGTC23.9215686274509802No Hit
GTGTCCCCACCCAAATCTCATGTGGAATTATAATTACCAATGCTGGGGGG11.9607843137254901No Hit
AGGCTGCCCGACGTCACCGTCGAGTTGAGCCCTGGCATTACCGCCATGCA11.9607843137254901No Hit
CTGCTGCGCGAGCGCACCGGTATGGGGTGCGCAAAGCTGGTGCACGGGGT11.9607843137254901No Hit
GAAAAACTATAGTTAAATGCACATACTAAATTATATTTAATAAAAATGTC11.9607843137254901No Hit
GGCCAAGCGCATCTGTGAGTCCTGTGAGGTCCGCCAGGAGTGCTTGGAGT11.9607843137254901No Hit
GAGTCACGAAGTCTCTCCACGACATGCCGGGCGTCGTCGTCAAGACTCTC11.9607843137254901No Hit
ATTTTATTGTTTCAAGTGTACAGAAGCTACTGTAGACAGTAGGTTGAAAA11.9607843137254901No Hit
ACAGTGCCCCTACCCCGACGCCTCGGACAATGAAATGCTCGACGGCCAAG11.9607843137254901No Hit
GGACAGATAGGATCACATCAAATTAAAACGTTTCTGCACAGCAAAGGATA11.9607843137254901No Hit
CTTAAAAGGTTTTGTTACAACACCAATCGTAAGTGCACCCATACTCTTAG11.9607843137254901No Hit
CGAGGAGTGATATATGTTGCTGGGCTGCTGGCCTGTCTCTACATCGCCCG11.9607843137254901No Hit
ATCTCTATCTGTACCCCAACGGCAGCCGACTCGACGACTCCATTGAAGCC11.9607843137254901No Hit
GACCGTCCCAGGATCATCGTGGCCGAGACGTGGTACAGCCCGTATCCGCC11.9607843137254901No Hit
TCACCAAGAAGAGTGCCCAGTTCGTCGCCGGTAAGCATCCGCCAGTCCCC11.9607843137254901No Hit
ACAGTGCCCCTACCCCGACGCCGCGGACAATGAAATGCTCGACGGCCAAG11.9607843137254901No Hit
CTGTGTGGTGGAGTTGATGCGAGTGAGAATGCAGGCATGAGTAGCGTGTG11.9607843137254901No Hit
TATGTGATGAGGACGAGATCGCACTGCTCAGTGCTCGATGAGCTCCAGTC11.9607843137254901No Hit
ACCCAGGTTTGGGCCCTCTAGATGGAGGTACTACCCTACTTCCTGCTGGA11.9607843137254901No Hit
GAGAAACCCCATCTCTACTAAAAATACAAAATTAGCCAGGCGTAGTGGTG11.9607843137254901No Hit
AGTCTGTACATAACATCAACAGTTAAACACTTCAAAAACTGCTTCAAGAT11.9607843137254901No Hit
CAGACCGACCACAAGGCCACCGTCAGGTACGTCAACTCAGTTGTTGCCAC11.9607843137254901No Hit
TTTCGGGAGGAACCACCCCGCCATGGAAACCCGGCCACCATTTCTCTGTG11.9607843137254901No Hit
GGCCACGTCTCGGCGGGTGACCTTGAGTAGTCCGTCGGCGAGCCTCGATG11.9607843137254901No Hit
TCTTGGTCTATGTCGATCTTGTCACCCTTGCTCGATTGGCTAACCAGTCA11.9607843137254901No Hit
AGGATGTGACGATCGGTGGCTTCGAGGATCGGCGACCGATCCTCATCCTC11.9607843137254901No Hit
TTATTAACGTCTGGATCGATACCAGCTCGCAACTTGTCGACGCCACGCAC11.9607843137254901No Hit
AATATCCTCCCAGGCGACCCCGGCATACAGATGCACCGGATCGGCCGAAG11.9607843137254901No Hit
GGTACATGTCGGTCGTCATGCTGCCGCCGTCAGTCTTGCGGTAGTTCCAT11.9607843137254901No Hit
CTCTTCAAGGCTTCATCTCCTGCAAGGACGGCGAATGCGCCTGTCTCTTA11.9607843137254901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)