FastQCFastQC Report
Tue 10 May 2022
EGAF00003668506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003668506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684880
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC35240.5145426936105595No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC32500.4745356850835183No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT29970.4375949071370167No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT26120.381380679827123No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT24430.35670482420278005No Hit
GTACATGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAA21680.31655180469571315No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG21100.30808316785422263No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA20980.30633103609391427No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA19860.2899778063310361No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC19150.2796110267492115No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC18780.2742086204882607No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT16790.24515243546314683No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA16540.24150216096250438No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG16260.23741385352178485No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC16140.23566172176147648No Hit
GTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGG15450.22558696413970328No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT15310.22354281041934354No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC15100.2204765798388039No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA15100.2204765798388039No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT14290.20864969045672233No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC14100.20587548183623408No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT14030.2048534049760542No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA13800.20149515243546315No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA13580.1982829108748978No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG13050.19054432893353582No Hit
GCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACC12520.1828057469921738No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA12490.1823677140520967No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG12190.1779873846513258No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT12120.1769653077911459No Hit
TAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTG11870.17331503329050343No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA11750.17156290153019507No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT11680.1705408246700152No Hit
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG11660.1702488027099638No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11650.17010279172993809No Hit
CCATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGG11590.1692267258497839No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA11570.1689347038897325No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC11280.16470038546898727No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT11200.1635322976287817No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG11120.1623642097885761No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10900.15915196822801075No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG10800.15769185842775377No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC10490.15316551804695713No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT10450.15258147412685436No Hit
ACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCG10400.15185141922672585No Hit
CTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGG10400.15185141922672585No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG10310.15053732040649456No Hit
ATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGAC10250.14966125452634038No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT10230.14936923256628898No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG10200.1489311996262119No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9830.14352879336526106No Hit
GTATCAACGCAGAGTACATGGGACATTTGCTTCTGACACAACTGTGTTCA9590.1400245298446443No Hit
CCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGG9580.1398785188646186No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG9370.13681228828407896No Hit
GAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGG9180.1340380796635907No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG9120.1331620137834365No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT9060.13228594790328232No Hit
TTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTT9050.13213993692325662No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGA9000.13140988202312814No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG8610.12571545380212593No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC8590.12542343184207452No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAG8590.12542343184207452No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA8580.12527742086204882No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT8580.12527742086204882No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC8440.12323326714168904No Hit
TTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAG8220.1200210255811237No Hit
GACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCG8210.11987501460109799No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG8190.11958299264104662No Hit
CTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCC8190.11958299264104662No Hit
CCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATG8170.11929097068099521No Hit
GTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG8110.11841490480084102No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC7990.11666277304053264No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCA7960.11622474010045554No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC7800.11388856442004439No Hit
CTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAAC7710.1125744655998131No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT7550.11023828991940195No Hit
GCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGG7530.10994626795935054No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC7490.10936222403924775No Hit
GTGCATCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAA7400.10804812521901647No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG7380.10775610325896506No Hit
CTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGA7370.10761009227893938No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT7360.10746408129891367No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA7360.10746408129891367No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG7300.1065880154187595No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT7290.10644200443873379No Hit
GAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGT7260.1060039714986567No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCT7210.10527391659852822No Hit
GCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCTTGTCACAGTGCA7180.10483588365845112No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA7150.10439785071837401No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA7110.10381380679827122No Hit
GAGTACATGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTC7050.10293774091811704No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG7020.10249970797803995No Hit
GCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGC7000.10220768601798855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)