FastQCFastQC Report
Tue 10 May 2022
EGAF00003668667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003668667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65881
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC5570.8454637907742748No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4400.6678708580622638No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA3110.4720632655849183No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC2760.41893717460269275No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT2610.3961688498960247No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG2410.3658110836204672No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA2360.35822164205157786No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT2350.35670375373779994No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG2340.35518586542402214No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC2300.34911431216891065No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG2210.3354533173449098No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT2160.3278638757760204No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC2070.31420288095201954No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT2050.3111671043244638No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT1940.2944703328729072No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1940.2944703328729072No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG1900.2883987796177957No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1800.273219896480017No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG1730.2625946782835719No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1720.261076789969794No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA1680.2550052367146825No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1620.2458979068320153No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG1600.24286213020445957No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA1590.24134424189068168No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA1540.23375480032179233No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT1540.23375480032179233No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT1440.21857591718401362No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT1420.21554014055645784No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA1410.21402225224267998No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT1370.2079506989875685No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC1360.2064328106737906No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT1340.20339703404623488No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA1310.19884336910490127No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG1300.1973254807911234No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC1260.1912539275360119No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA1260.1912539275360119No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG1250.189736039222234No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC1230.18670026259467828No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC1230.18670026259467828No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA1210.18366448596712254No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC1170.17759293271201107No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC1160.17607504439823318No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC1120.1700034911431217No Hit
GCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGAT1120.1700034911431217No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG1110.1684856028293438No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG1050.15937827294667659No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC1030.15634249631912084No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA1020.15482460800534298No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC990.15027094306400934No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG970.1472351664364536No Hit
AACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGA950.14419938980889785No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA930.14116361318134213No Hit
AACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTG930.14116361318134213No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC920.13964572486756424No Hit
CTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGC920.13964572486756424No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA910.13812783655378638No Hit
GTACATGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCA900.1366099482400085No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC900.1366099482400085No Hit
ATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGC900.1366099482400085No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG900.1366099482400085No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG900.1366099482400085No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT880.13357417161245277No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA870.1320562832986749Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGA850.12902050667111914No Hit
CGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACAT840.12750261835734125No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG820.12446684172978553No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT820.12446684172978553No Hit
GGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACAC820.12446684172978553No Hit
ATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCC810.12294895341600764No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA810.12294895341600764No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA800.12143106510222978No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT800.12143106510222978No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT790.1199131767884519No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT790.1199131767884519No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT780.11839528847467404No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA780.11839528847467404No Hit
GGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGC780.11839528847467404No Hit
ATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCT770.11687740016089616No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC770.11687740016089616No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC770.11687740016089616No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA760.11535951184711829No Hit
GAGTACATGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG750.11384162353334042No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA750.11384162353334042No Hit
CGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTC750.11384162353334042No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT750.11384162353334042No Hit
GTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGT750.11384162353334042No Hit
CTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTG740.11232373521956256No Hit
CTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTG730.11080584690578467No Hit
CTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTC730.11080584690578467No Hit
GTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCA730.11080584690578467No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA720.10928795859200681No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT720.10928795859200681No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA720.10928795859200681No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC720.10928795859200681No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGT720.10928795859200681No Hit
CGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGG720.10928795859200681No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG710.10777007027822892No Hit
GTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCC710.10777007027822892No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG710.10777007027822892No Hit
GTACATGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCAC710.10777007027822892No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG710.10777007027822892No Hit
GTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCG700.10625218196445105No Hit
TCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC700.10625218196445105No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG700.10625218196445105No Hit
ATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACATTCAAA690.10473429365067319No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA690.10473429365067319No Hit
GCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA690.10473429365067319No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA690.10473429365067319No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT680.1032164053368953No Hit
ACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGA680.1032164053368953No Hit
TTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGA670.10169851702311744No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA670.10169851702311744No Hit
GTACATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTC670.10169851702311744No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG660.10018062870933958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)