FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003668833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003668833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42.515723270440252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG31.8867924528301887Clontech Universal Primer Mix Long (96% over 26bp)
GTATTAGTGTTCCTATTATTATTATTTTATAGAAATAATGAATGTTAAGG21.257861635220126No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.257861635220126Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.257861635220126Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.257861635220126Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.257861635220126Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGT21.257861635220126Clontech Universal Primer Mix Long (95% over 21bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTG21.257861635220126Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTGGGTCTCCCTGGGGCTGTCAAGAGCTGCCCCAGCGTTCCCCTGCCT21.257861635220126No Hit
CCATGTACTCTGCGTTGATACCGAAGCAGTGGTATCAACGCAGAGTACAT10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTACACCTATTGCACCTATTATGCCCTGCCACTGGCAAGCTGAAAACT10.628930817610063No Hit
ATCTTTTGAACCGGACTATTGAAATTTAACTTAAATGTCAGAAATCTTTA10.628930817610063No Hit
GAGTACATGGGTCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTC10.628930817610063Illumina Single End PCR Primer 1 (100% over 36bp)
CTTCCCTACACGACGCTCTGAACTTGGGACAGAGCGTCGTGTAGGGAAGA10.628930817610063No Hit
ACTTTGCATTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTC10.628930817610063No Hit
GGTCCTGAAAAACGAGCGTCGTGTAGGGAGAGGTAGTTCGGGTCCGACCC10.628930817610063No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAA10.628930817610063No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTAT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGT10.628930817610063Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTC10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTGATCGGCACCAGATACACTCTTCCCTACACCTGTCTCTTA10.628930817610063No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTAAAAAAAAAAAAAAAAAAAAAAAA10.628930817610063No Hit
CTACAACCTAAACAAATGTAAAAAATCAAAAAAAATCTATTAAACACATC10.628930817610063No Hit
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTACACCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC10.628930817610063No Hit
CTTTTGACGGCGTGGTAGCGATTCACCAGCGCCACCGCGAGGTCGGCCGG10.628930817610063No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGATGGCTCT10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGTGGGTGGATCACTTGAGGTCAGGAGTTCGAGACCTGTCTCTTATAC10.628930817610063No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACTCAGATTTTATACA10.628930817610063No Hit
CGCCAATGCTGGGTGCGCACTCGCCGATCGCCGTCCCACCCATAGCAGGT10.628930817610063No Hit
CGATTAAAATCGGCCAACGCACGAACGGTCTTGGACAACACGGTTACCAG10.628930817610063No Hit
GAAGAGCGTCGTGTAGGGAAAGATACTCGGGCTTACCATGTACTCTGCGT10.628930817610063Illumina Single End PCR Primer 1 (95% over 23bp)
TCCTCCAGAAGACCATTTTCTGGGAGGTGCCCTGGATCCAGCTCAGCATC10.628930817610063No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGC10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GATGATAGGCGCCCCGAGTCTACACTCTTCCCTACACCTGTCTCTTATAC10.628930817610063No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCCTGTCTCT10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCAAATGGAATCGAATGGAATCATCATCGAATGGAATCTAATGGAATA10.628930817610063No Hit
CTATTTGATGATTCCTTTCAATTCCATTCAATTTTTCCTGTCTCTTATAC10.628930817610063No Hit
GTACATGGGAAGCAGTGGTATCAACGCTGAGTACATGGGAAGCTGTCTCT10.628930817610063Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.628930817610063Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCGCGTGTAGGAAAGAGTGAGTCTCGGTGCGTCCCATGTA10.628930817610063No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAACCCAGGGTGCCCA10.628930817610063No Hit
GTACATGGGATGATCGGCGACCACCGAGATCTACCTCTGTCTCTTATACA10.628930817610063Illumina Single End PCR Primer 1 (95% over 21bp)
GATTTAAATTCCTTGTTGACTACCTGGTATAGTGACCTACAGAGTTGCTT10.628930817610063No Hit
GCCTTGACCTTCTCGGGGGCGGCATTGAAGGTGATGTCCGTTGCCCCGGC10.628930817610063No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTAT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GGATTGGCCTATCTAAGTTAAAGTGATCAATTCAGAGGTAGGCACATGAC10.628930817610063No Hit
TTCCCAGGCTGGTCTCGAACTCCTGGCCTCAAGTGATCCTCCCAATTTGG10.628930817610063No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTGGATACCACTGCTTCCC10.628930817610063No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGTGCGACACCGAGACTACACTCTTTC10.628930817610063No Hit
GTGGTATCAACGCAGACTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACA10.628930817610063Clontech SMART CDS Primer II A (96% over 25bp)
GAATTATCATGAAATTGAAATGGATGGACTCATCATCGAATGGATTCGAA10.628930817610063No Hit
GTACATGGGAAGATACGGCGACCACCGATATCTACACTCTTTCCCTACAC10.628930817610063Illumina Single End PCR Primer 1 (97% over 39bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAGGCACCACC10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCTCCAGCAGCACGGCGCCGGCGCCGTCGGAGAGCATCCAGCGCAGGAA10.628930817610063No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTCTTATAAACA10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATAACAACAGAACCAGAAAAAACAACTCACACTAAAACACAACAACTAG10.628930817610063No Hit
TTCTCGTTTGTTGTTTTTGGGTTTAAAGGTGAGTTTTTGAACTTACATCT10.628930817610063No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.628930817610063Clontech SMART CDS Primer II A (100% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.628930817610063Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGACGGCCGAGTCCTCTTTCCTACACGACGCTCTTCCGATCCT10.628930817610063Illumina Single End PCR Primer 1 (96% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
CAGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGCTGTCTCTTATACA10.628930817610063Illumina Single End PCR Primer 1 (96% over 30bp)
ATACTTAACGTTGGCGCTAATGGGCGGTGCCGCTTTTTTCGTATTGAAAG10.628930817610063No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTA10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
TTTCACTAAACGACAATAAAACGAACATTTCTCTTATACACATCTCCGAG10.628930817610063No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGG10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GAGATGTGTATAAGAGACAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.628930817610063No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
CCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACC10.628930817610063Illumina Single End PCR Primer 1 (100% over 42bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.628930817610063Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.628930817610063Clontech SMART CDS Primer II A (96% over 26bp)
GTCGTGTAGGGAAAGAGTGTAGATCTCGTGGTCGCCACATCCCATGTACT10.628930817610063Illumina Single End PCR Primer 1 (96% over 28bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT10.628930817610063No Hit
AATCATACAACAACTGAAGAAGATGAAAAAACTCATATAAAAATCTAATA10.628930817610063No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGGAAAGAGTGTAGACTCGGTGGTCGCCGTCTCCATGTACTCTGC10.628930817610063No Hit
GTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACATTCCCATG10.628930817610063Illumina Single End PCR Primer 1 (97% over 40bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTCATTTATAACA10.628930817610063No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTCGGGCTGCTGACGCTGGACCTGGTGCGCGTCGTCGATGCCAATGC10.628930817610063No Hit
CCACGAGGCCTCGTCGTCGAAGACCTTCACTTCCTTGAGCTTGGCCAGCG10.628930817610063No Hit
GAGTACATGGGATGGACCACCGATCACATCTTTCCCAACACGACGCTCTT10.628930817610063Illumina Single End Sequencing Primer (95% over 22bp)
GGAAGCAGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.628930817610063No Hit
TCTCGGTGGTCGCGTATCATCCCATGTACTCTGCGTTGATACCACTGCTT10.628930817610063Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACA10.628930817610063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.628930817610063No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTCTTATACACA10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCC10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCTGACTCAAAACAAAACAAAACAAACAAACAAACAAACAAAAAAGAA10.628930817610063No Hit
CAGTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CTGCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA10.628930817610063Clontech Universal Primer Mix Long (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTTCTTTGAGATCTCGTATG10.628930817610063No Hit
CAAAAAAAAAAAAAAAAAAAAAAGAAAACTAAACAGCGGTTGATACAAAA10.628930817610063No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA10.628930817610063Clontech Universal Primer Mix Long (95% over 22bp)
ATTTTCACGCGATTACCTCCGCAACCAACACCTTGGCCTCCATCATTGAT10.628930817610063No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGCACTCTGCGTTGATACCAC10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCCTGCCTGTCT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCAGCGTTGAT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGCTGTCTCTTATACACATCTCCG10.628930817610063No Hit
TCCCTACACGACGCTCGTTTTTTCAGGACCTGTTGGTACCAGATCGGAAG10.628930817610063No Hit
AGCCAAGACAGCCACAGAGCACAGCGGGAACACGACTGGCACAACACAAC10.628930817610063No Hit
CGTCTCTACTAAAAATACAAAAAATTAGCCGGGCGCGGTGGCGGGCGCCT10.628930817610063No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAGTAC10.628930817610063Clontech Universal Primer Mix Long (95% over 21bp)
GTGTAGGGAAAGAGTGTAGACTCGGTGGCGCCGTATCACCCATGTACTCT10.628930817610063No Hit
GATGCAGTGGCCTCTTCAAGTCCCCTGCACACCCCCACCCATGCATCCTG10.628930817610063No Hit
GTACATGGGAATGATACGGCGACCACCGAGATCTACACTCTTTGGGGAGA10.628930817610063Illumina Single End PCR Primer 1 (97% over 34bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAATCAAAACAGATAACATCAGAAACAGATGTATCAAATAAAAATAAAAG10.628930817610063No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGTATACGCACCC10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
CACCGCCGCCGCGCCGTGGCGCTGGAACCAGTCCCGCGCATTGTCGATGT10.628930817610063No Hit
CTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCA10.628930817610063Illumina Single End PCR Primer 1 (96% over 30bp)
GTGTAGTTCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATA10.628930817610063Illumina Single End PCR Primer 1 (96% over 29bp)
GTATCATTGCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG10.628930817610063Clontech SMART CDS Primer II A (96% over 26bp)
GGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAGAGCGTCGTGTAG10.628930817610063No Hit
GGTGGAGGCCGCGGCGATGGCCGTGGCCTCGTCGTTCGGGCGCTGCGCCA10.628930817610063No Hit
GTACATGGAATACGCGACACATCTACACTTACCTACACTACGCTATTCCG10.628930817610063No Hit
ATCACGATGATGATGGCTACCAAGAACACGAGAACAGAAGCCTTGGCACC10.628930817610063No Hit
GGGAAAGAGTGTAACTCGTGGTCGCCGTACATTCCCATGTACTCTGCGTT10.628930817610063No Hit
GTGATGCCCCAGCGCTCCAGTTCGATCTTCTCGTCGAGGATGGGTTCGCG10.628930817610063No Hit
CTCTCACCCTCCTGCTCCTCAACCTGGTGGTGAAAGCTGCCAGGACACTG10.628930817610063No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACACTGCTTTTAACCC10.628930817610063No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.628930817610063Clontech Universal Primer Mix Long (95% over 21bp)
GTGTAGGAAAAGGTAGTCCGCCCCATGTACTCTGCGTTGATACCACTGCT10.628930817610063Clontech SMART CDS Primer II A (96% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGATATACGCGACCACCGTCTACAT10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 22bp)
TCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCCTGTC10.628930817610063Illumina Single End PCR Primer 1 (100% over 22bp)
GAACAGGAAGACACTGGTTTAAACCAGACTCTAGTAATTATAAAACTTAG10.628930817610063No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTT10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAAGAGTGTAGATCTCGGTGTCGTATTCCCATGTACTCTGCGTTGATACC10.628930817610063No Hit
TAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACATTCCCATGTACCTG10.628930817610063Illumina Single End PCR Primer 1 (97% over 34bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTT10.628930817610063Clontech Universal Primer Mix Long (95% over 21bp)
GAGTTACACTCTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTC10.628930817610063Illumina Single End Sequencing Primer (96% over 27bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA10.628930817610063No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)