FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003668959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003668959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATTATCCTGGATTGTCTCCGTGGGCATTAAATCCTGGCACATATATCC38.571428571428571No Hit
ATGCCTTCCCTCCATGTTCCATCACGGCGCCACAATCTGTGTCCCTTAAC38.571428571428571No Hit
TATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGC25.714285714285714No Hit
CCTTGTATACCCAATACAGGTTCACCATCATTAATAGCAAACAAATGGGA25.714285714285714No Hit
CTGGGGGAGTGGGCCAAGCCTGAGAGAGGAGGGCCCTGCTCCAGAGATCC25.714285714285714No Hit
TCCCGAGGCACAGGAAGCTCTGGGCCCTCCTCTCAACATCCATTATCTCA25.714285714285714No Hit
TGCACAGGGTTGTCTCATTGAAGCGGAGCACGCATGAGTGCCCCCCGACC25.714285714285714No Hit
GGGATCGGGGATTGCAATTATTCCCCATCACGAATGGGGTTCAACGGGTT25.714285714285714No Hit
GCCTGGACCCAGTGGCCAGCTGTCCTGGACAGTGACAGCCACAGAACCCT12.857142857142857No Hit
CTCCACTGCTCCGCCACACGGGCCGCCTCCCACTTGCGCTGGGTGATCTG12.857142857142857No Hit
GCCTAATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTA12.857142857142857No Hit
CCCCCTTGCCCGTTCTGTCCAGGCCGTAGGCGATGGCGGCGGCCGTGGGC12.857142857142857No Hit
GCTGAGACCCAGCCCAGGTAGAAGGACCAGGAGAAGAAGGTCTGGATCTG12.857142857142857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG12.857142857142857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTACCATGAAGCAAGAAAAGACCAAGGAGATGGTGAAGACAGCAGAAG12.857142857142857No Hit
GACCTGTACGCCAACACAGTGCTGTCTGGCGGCACCACCATGTACCCTGG12.857142857142857No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC12.857142857142857No Hit
TTGCTCCCCCAGTGGATTGGGTGGCTCCATTCACTCCAATGCTGAGCACT12.857142857142857No Hit
GTGAGAGACACATCAGAGCCCTGGGCACTGTCGCTGCACGCAGCCTGAGA12.857142857142857No Hit
ATCTTGATCTTCATTGTGCTGGGTGCCAGGGCAGTGATCTCCTTTTGCAT12.857142857142857No Hit
CGCTAGAGGTGAAATTCTTGGACCGGCGCAATACGAATGCCCCCGGCCGT12.857142857142857No Hit
AGGGTGCAGCGGTCCTTGACCTCCTCGTAGCAGTTTGCTTGCAATTCATG12.857142857142857No Hit
GGATGGTTCCTTGCCATTGAAGAACTCTTTAACCAGTTGCTGAATCTTTG12.857142857142857No Hit
GGTGACCTGTTGACCAAAACACAGGAGCTGGGCCGTGACTACAGGACCTG12.857142857142857No Hit
GTGCCCACTTCACTAAAAACTCTACATCAATGTCCCAGGAGCCCGGAGGC12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)