FastQCFastQC Report
Tue 10 May 2022
EGAF00003669028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10641
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1791.6821727281270558Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1681.5787989850577955Clontech Universal Primer Mix Long (96% over 26bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1391.3062682078751997No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC710.6672305234470445Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG590.5544591673714876Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG570.5356639413588948Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT550.516868715346302No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC540.5074711023400056Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT540.5074711023400056Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT420.39469974626444887No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC390.3665069072455596Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG330.31012122920778123No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG320.3007236162014848No Hit
GCCCCACACCGCGCCGGAGGACGGGTCGTTGAGCCCCGAAACCACCGTCC320.3007236162014848No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC320.3007236162014848No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA320.3007236162014848No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC310.2913260031951884Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG300.281928390188892Clontech Universal Primer Mix Long (96% over 26bp)
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC290.2725307771825956No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG290.2725307771825956No Hit
CGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGG290.2725307771825956No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG280.26313316417629923No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT270.2537355511700028No Hit
GCCTAGTACCGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC270.2537355511700028No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG270.2537355511700028Clontech Universal Primer Mix Long (96% over 26bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA270.2537355511700028No Hit
GTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACT260.24433793816370641No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG250.23494032515741No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT250.23494032515741No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC240.22554271215111363Clontech Universal Primer Mix Long (96% over 25bp)
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT240.22554271215111363No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC230.21614509914481722Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC230.21614509914481722No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG220.20674748613852081Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT220.20674748613852081No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT220.20674748613852081No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC200.18795226012592803No Hit
CAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACG200.18795226012592803No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA200.18795226012592803No Hit
GTGCAGCACCTACCGCGGCAGCGCGCGCGACTGGATGTCGAAGGGCACGC190.17855464711963162No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT190.17855464711963162Clontech Universal Primer Mix Long (100% over 23bp)
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG190.17855464711963162No Hit
CGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTC180.16915703411333521No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA180.16915703411333521No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA180.16915703411333521No Hit
CCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCC180.16915703411333521No Hit
GTCTACGTGAGCCTCGTGGCGGCGGCGCCCTACGTGCTGCGGATCGGCCT180.16915703411333521No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG170.1597594211070388No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA170.1597594211070388Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC170.1597594211070388No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT160.1503618081007424No Hit
CACTAGAGCGACACCAACATCGTTACGCTTACACACCGGACGCTTGGATC160.1503618081007424No Hit
GCCCTATTACGAGGGTTGTAGTCGTGTGGCACCAGCGCGGCTACTCCGAT160.1503618081007424No Hit
ACATGGCAGTTGGCCCACATGGAATATCGGTTTGAGGCCCCGAGCTGGAG160.1503618081007424No Hit
GCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGG160.1503618081007424No Hit
AGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGC160.1503618081007424No Hit
GGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACAC160.1503618081007424No Hit
GTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCC160.1503618081007424No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG160.1503618081007424No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG150.140964195094446No Hit
GTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGT150.140964195094446No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT150.140964195094446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGT150.140964195094446No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA150.140964195094446No Hit
ATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGC140.13156658208814961No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC140.13156658208814961Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA140.13156658208814961Clontech SMART CDS Primer II A (96% over 26bp)
CTGTGGATCTGCTCCGGCAACATCGGCTTCACCACCGTGCCATCGACGCC140.13156658208814961No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG140.13156658208814961Clontech Universal Primer Mix Long (96% over 26bp)
GCCTAGTACGCGCGGGGCTCGAAAGGTACTAGGCCCTTATAGGGGACGAA140.13156658208814961No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA140.13156658208814961Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCCCTTATAGGGGACGAACCCGGGGCTGCGCGTGACGAACCAC140.13156658208814961No Hit
CCGTAGGGGCCCTCGCCGAAGGCATTGCTCTCGAACAGCACGAGCCGCCC140.13156658208814961No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC140.13156658208814961No Hit
CAACATGGCAGTTGGCCCACATGGAATATCGGTTTGAGGCCCCGAGCTGG140.13156658208814961No Hit
CTACAAGGGCCTGAACGGCATCGCCATCGACGGCGGGCGCGATGTCTACG140.13156658208814961No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA140.13156658208814961No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT140.13156658208814961No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA130.12216896908185321No Hit
GTTCTTATCTGCCTAGTACGCGCGGGGCTCGAAAGGTACTAGGCCCTTAT130.12216896908185321No Hit
GGTCCCTACAAGGGCCTGAACGGCATCGCCATCGACGGCGGGCGCGATGT130.12216896908185321No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA130.12216896908185321No Hit
GTACATGGGTCCCGCCCCACCGCAATTTCCATGCGCATATGAGTGCTATA130.12216896908185321No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG130.12216896908185321Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG130.12216896908185321No Hit
GTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTC130.12216896908185321No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA130.12216896908185321No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG130.12216896908185321No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG130.12216896908185321No Hit
CCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCTAT130.12216896908185321No Hit
CACGTAGACATCGCGCCCGCCGTCGATGGCGATGCCGTTCAGGCCCTTGT130.12216896908185321No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGT130.12216896908185321No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG130.12216896908185321Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA130.12216896908185321Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCC130.12216896908185321No Hit
ATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTG120.11277135607555681No Hit
GTCTATACATGGACGACACTTTGCACATCATAGTGCGACGTTGATCCAGA120.11277135607555681No Hit
CTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTC120.11277135607555681No Hit
GTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTC120.11277135607555681No Hit
AACATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCC120.11277135607555681No Hit
GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG120.11277135607555681No Hit
CTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTG120.11277135607555681No Hit
CCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGC110.10337374306926041No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT110.10337374306926041No Hit
CCCCTCGTTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCC110.10337374306926041No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA110.10337374306926041No Hit
CTATAAGCGCTGTCACGGATGTATAAATCGCTCGAGAATCACACACAGTC110.10337374306926041No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC110.10337374306926041No Hit
GCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGA110.10337374306926041No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG110.10337374306926041No Hit
TCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC110.10337374306926041No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT110.10337374306926041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)