FastQCFastQC Report
Tue 10 May 2022
EGAF00003669043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196429
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT5640.2871266462691354No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA4430.22552678066884219No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC4090.2082177275249581No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC3940.20058138054971517No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC3930.20007229075136562No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT3840.19549048256621987No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3800.19345412337282172No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC3770.19192685397777315No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG3630.1847995968008797No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT3570.18174505801078253No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT3450.17563598043058815No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC3420.17410871103553954No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG3350.17054508244709285No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG3130.15934510688340317No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA3100.15781783748835457No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG3040.15476329869825736No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2950.1501814905131116No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC2770.14101787414282005No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT2760.14050878434447053No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC2730.13898151494942193No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT2710.13796333535272287No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC2680.1364360659576743No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG2640.13439970676427615No Hit
CTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAG2600.13236334757087803No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC2590.1318542577725285No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2570.13083607817582943No Hit
CCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTG2560.1303269883774799No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA2520.1282906291840818No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG2500.12727244958738274No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT2480.12625426999068368No Hit
TTCATCGACCTCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGG2410.12269064140223694No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT2390.12167246180553788No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC2340.11912701281379023No Hit
GTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTC2310.11759974341874163No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC2300.11709065362039212No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT2270.11556338422534351No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC2230.11352702503194538No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC2210.11250884543524632No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA2200.1119997556368968No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG2190.11149066583854726No Hit
CTCATTCATCGACCTCCCCACCCCATCCAACATCTCCGCATGATGAAACT2180.11098157604019773No Hit
ATTCTTTACCTTTCACTTCATCTTGCCCTTCATTATTGCAGCCCTAGCAG2170.1104724862418482No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT2140.10894521684679961No Hit
GTCTTTGATTGTGTAGTAAGGGTGGAAGGTGATTTTATCGGAATGGGAGG2120.10792703725010055No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC2060.10487249846000336No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT2060.10487249846000336No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT2050.10436340866165382No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTA2010.1023270494682557No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC2000.10181795966990619No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA2000.10181795966990619No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT1990.10130886987155664No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC1990.10130886987155664No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA1980.10079978007320713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)