FastQCFastQC Report
Tue 10 May 2022
EGAF00003669426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572631
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG51800.9045965028089643No Hit
AGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT48220.8420780572480357No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC41420.7233279371881718No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG38620.6744308289282278No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT36450.6365355700267712No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG34630.6047524496578076No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT34430.6012597990678116No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA33480.5846697087653305No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC25300.4418202996344941No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA24810.43326330568900395No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC24730.4318662454530055No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA23410.4088147515590319No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT21910.3826198721340619No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC20950.36585514930208113No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC19210.3354690891691159No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG18860.3293569506366229No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA18310.3197521615141339No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT18080.3157356133356385No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG18000.3143385530996401No Hit
CTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTC17710.3092742097441459No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC17420.3042098663886517No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC16540.2888422037926693No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG16070.28063447490617865No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA15690.27399843878518626No Hit
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA15660.2734745411966869No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGC15660.2734745411966869No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT15150.264568282192197No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT14760.25775761354170484No Hit
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT14690.25653518583520624No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG14480.25286790271571047No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT14400.2514708424797121No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA14040.24518407141771925No Hit
TCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACT13990.24431090877022024No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT13940.2434377461227213No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG13610.23767487264922788No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT13480.23540464976573044No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA13400.23400758952973208No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC13360.23330905941173286No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT12790.22335500523024426No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG12670.22125941487624667No Hit
CACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCT12620.22038625222874766No Hit
CTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCT12540.21898919199274924No Hit
CAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGG12400.21654433657975208No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG12240.21375021610775527No Hit
GGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGC11860.20711417998676285No Hit
GTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATC11510.20100204145426986No Hit
GTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGC11350.19820792098227308No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC11230.19611233062827543No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC10760.18790460174178486No Hit
AGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACACCCTCCAGTTA10610.18528511379928783No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC10520.18371342103378965No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC10500.18336415597479003No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC10460.18266562585679086No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC10040.17533105961779924No Hit
CTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGA10010.17480716202929986No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC9980.17428326444080044No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT9860.17218767408680283No Hit
GGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATG9800.17113987890980403No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC9780.17079061385080443No Hit
AGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT9690.16921892108530623No Hit
GAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG9560.16694869820180883No Hit
TGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGAT9550.16677406567230904No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC9540.16659943314280926No Hit
CTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGT9500.16590090302481003No Hit
GCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTT9470.16537700543631065No Hit
ACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGA9430.16467847531831145No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT9380.16380531267081244No Hit
TGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGA9270.16188435484631464No Hit
GCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACAC9100.15891560184481804No Hit
CTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCG8990.15699464402032023No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC8950.15629611390232104No Hit
TATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAA8950.15629611390232104No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG8950.15629611390232104No Hit
CTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTT8890.15524831872532224No Hit
CCTCATAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGA8760.15297809584182484No Hit
GCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGA8030.14022992118833943No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG7980.13935675854084043No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA7890.13778506577534225No Hit
GCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAG7680.13411778265584642No Hit
TCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCC7640.13341925253784723No Hit
TAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGAAAGCT7480.13062513206585044No Hit
CTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCT7410.12940270435935183No Hit
GAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTT7360.12852954171185285No Hit
CCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTC7320.12783101159385363No Hit
CCCTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTG7270.12695784894635462No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG7230.12625931882835542No Hit
GACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCA7210.12591005376935582No Hit
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC7070.12346519835635864No Hit
CTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATG7040.12294130076785922No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT7040.12294130076785922No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG7000.12224277064986004No Hit
ATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC7000.12224277064986004No Hit
GGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGG6990.12206813812036023No Hit
ACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC6940.12119497547286122No Hit
CTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATG6850.11962328270736303No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA6850.11962328270736303No Hit
TTTGCGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC6830.11927401764836341No Hit
GTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGC6800.11875012005986402No Hit
CTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCC6720.11735305982386564No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA6700.11700379476486604No Hit
GCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAAT6670.11647989717636664No Hit
TTTTCGAGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGG6650.11613063211736703No Hit
CTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGC6630.11578136705836742No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG6610.11543210199936785No Hit
CCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGG6540.11420967429286923No Hit
GCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCAC6430.11228871646837144No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG6280.10966922852587443No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA6250.10914533093737502No Hit
CTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCC6230.10879606587837544No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG6060.10582731287687884No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG5960.10408098758188083No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT5900.10303319240488203No Hit
GTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGC5800.10128686710988402No Hit
CTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATCAGGGCTGAAAAGTT5780.10093760205088442No Hit
CAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGG5760.10058833699188484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)