FastQCFastQC Report
Tue 10 May 2022
EGAF00003669439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141836
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5390.38001635691925884No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA4290.3024619983643081No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC4240.29893680024817393No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3970.2799007304210497No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA3850.27144025494232776No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC3810.2686200964494205No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA3810.2686200964494205No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA3510.24746890775261568No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3480.24535378888293521No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT3460.2439437096364816No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3440.24253363039002793No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG3400.23971347189712064No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT3310.23336811528807921No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT3170.22349756056290362No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG3120.21997236244676951No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA3090.21785724357708905No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA3040.2143320454609549No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG2730.19247581714092332No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG2640.1861304605318819No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC2600.18331030203897458No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT2590.1826052624157478No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT2570.18119518316929412No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2520.17766998505315998No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG2420.17061958882089173No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC2320.16356919258862349No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT2290.161454073718943No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT2260.15933895484926253No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA2200.1551087171099016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.14664824163117968No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC2020.14241800389181872No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1930.1360726472827773No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1880.13254744916664316No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC1810.1276121718040554No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG1810.1276121718040554No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA1810.1276121718040554No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA1770.12479201331114809No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC1760.12408697368792125No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA1730.1219718548182408No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG1710.12056177557178713No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA1670.11774161707887984No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG1660.117036577455653No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA1660.117036577455653No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT1630.11492145858597252No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT1610.11351137933951888No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT1600.11280633971629205No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA1540.1085761019769311No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT1540.1085761019769311No Hit
TTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCT1540.1085761019769311No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG1510.10646098310725062No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1500.10575594348402381No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG1490.10505090386079698No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1470.10364082461434333No Hit
GGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCC1470.10364082461434333No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG1470.10364082461434333No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC1460.1029357849911165No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC1440.10152570574466285No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT1430.10082066612143602No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG1420.10011562649820921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)