FastQCFastQC Report
Wed 8 May 2024
EGAF00003669784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121399
Total Bases12.1 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG8600.7084078122554551No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT5110.4209260372820205No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC4890.4028039769685088No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC4830.39786159688300565No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT4770.3929192167975024No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT4520.3723259664412392No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA4150.3418479559139696No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA3980.3278445456717106No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT3450.2841868549164326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.25865122447466615No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC3090.25453257440341354No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT3090.25453257440341354No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC2900.23888170413265347No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA2480.20428504353413127No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT2480.20428504353413127No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGT2450.20181385349137965No Hit
GTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAG2170.17874941309236483No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG2140.17627822304961327No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG2110.17380703300686168No Hit
GAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTT2050.1688646529213585No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT1960.16145108279310372No Hit
GTACATGGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACC1950.1606273527788532No Hit
GACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC1880.15486124267909948No Hit
GTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAAC1880.15486124267909948No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACAT1740.1433290224795921No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGT1720.14168156245109104No Hit
ACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCG1670.1375629123798384No Hit
GTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT1600.13179680228008467No Hit
CCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCC1590.13097307226583416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.12767815220883202No Hit
CCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCC1540.12685442219458148No Hit
GGAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTT1540.12685442219458148No Hit
AGTCAAAGCTGTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATA1530.12603069218033097No Hit
CTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCA1500.12355950213757938No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC1500.12355950213757938No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG1500.12355950213757938No Hit
GTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGG1480.12191204210907833No Hit
TGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTG1460.12026458208057728No Hit
GAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAAC1450.11944085206632674No Hit
CTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTG1450.11944085206632674No Hit
CGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT1420.11696966202357514No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT1370.1128510119523225No Hit
CCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTC1350.11120355192382145No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC1340.11037982190957092No Hit
GAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTT1320.10873236188106987No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACAT1300.10708490185256879No Hit
GGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCCCAGCA1280.10543744182406774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)