FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003669789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22.1739130434782608No Hit
GTATCAACGCAGAGTACATGGGACGGCGACCACCGAGACTGTCTCTTATA22.1739130434782608No Hit
GCCCTACCGCGAGGACGACGCCACCGCGCCCCAGGGCGTCTATGGCCGCA22.1739130434782608No Hit
GAAGCAGGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGT22.1739130434782608No Hit
GGGCCGACGGGCCTCATGCTCGCCGGCGAGCTGGCCCTGGCAGGCGTCGA22.1739130434782608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT22.1739130434782608No Hit
GAAAAAAAAAGTGTAGATCTCGGTGGTCGCCCCATGTACTCTGCGTTGAT22.1739130434782608Illumina Single End PCR Primer 1 (96% over 26bp)
GCAGAGTACATGGGTGGCGACGGCAGCGAGGCGAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
ACGCAGAGTACATGGGTGTCGGCGACCACCGATCACACTCTTTCCCTAAA11.0869565217391304No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAAAC11.0869565217391304No Hit
CAAACTCCTGACCTCAGGTGATCCACCTGCCTCAGTCTCCCAAAGTGCTG11.0869565217391304No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
GTAGGAAAGAGTGTAACTCGGTGTCGCCGTTCACCCATGTACTCTGCGTT11.0869565217391304No Hit
TCAACGCAGAGTACATGGGACGGCGACCACCTGTCTCTTATACACATCTC11.0869565217391304No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAAGCGTTTGAATGTGTGA11.0869565217391304No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTATAA11.0869565217391304No Hit
GGGAAAGAGTGTAGATCTCGGTGGTCGCGACACCCATGTACTCTGCGTTG11.0869565217391304Illumina Single End PCR Primer 1 (96% over 28bp)
ATCGTGCCAGTGCACTCCAGCCTGGGCGACCTGTCTCTTATACACATCTC11.0869565217391304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0869565217391304No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCAAAAAAAAAAAA11.0869565217391304No Hit
GACCTCCACGCCGCCCGGTATGCCTACTTCGTGGCCCTCATAGTTCCGTT11.0869565217391304No Hit
TATCAACGCAGAGTACATGGGACGGCGACCACCGAGATCCTGTCTCTTAT11.0869565217391304No Hit
AAAGTGTAGATCTCGGTGGTCCCCATGTACTCTGCGTTGATCTGTCTCTT11.0869565217391304Illumina Single End PCR Primer 1 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGAAC11.0869565217391304No Hit
CGTCAGATGTGTATAAGAGACAGGGAAAAAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGG11.0869565217391304No Hit
GAGCTGACCAAGCGGCACGACCTGCCCTACCACGTGGTGGGCTCCGACCT11.0869565217391304No Hit
GTACATGGGTGAACGGCGCACCGAGTCTACACTCTTTCCCTACACCTGTC11.0869565217391304Illumina Single End PCR Primer 1 (95% over 21bp)
GTGGTATCAAGCAGAGTACTGGGAGCAGTGGTATCAACGCAAAAAAAAAA11.0869565217391304No Hit
GTGTAGGGAAAAGTGTAGATCTCGGTGCGCCGTACTCCCATGTACTCTGC11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAACACA11.0869565217391304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC11.0869565217391304No Hit
TCAACGCAGAGTACATGGGCGGCGACCACTGAGATCTACCTGTCTCTTAT11.0869565217391304No Hit
CCCATCGTCGTCATGACGACCGAGGTGCTGCGCAACATCTGTCTCTTATA11.0869565217391304No Hit
GCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACG11.0869565217391304No Hit
GATCTCGGTGGTCGCCGTACCCATGTACTCTGCGTTGATACCTGTCTCTT11.0869565217391304Illumina Single End PCR Primer 1 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTT11.0869565217391304No Hit
GTATCAACGCAGAGTACATGGGACGCGACCCGAGATCCTGTCTCTTATAC11.0869565217391304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.0869565217391304No Hit
GTGTATAAGAGACAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAC11.0869565217391304No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAGTACTCCTGTCACTTATAAAAA11.0869565217391304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCA11.0869565217391304No Hit
AAAGTGTAGATCTCGGTGGTCGCCGTCCCATGTACTCTGCGTTGATACCT11.0869565217391304Illumina Single End PCR Primer 1 (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC11.0869565217391304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAACCACCCAATTAA11.0869565217391304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAG11.0869565217391304No Hit
GCTTGACGGCATCGGCGGCCGCGGGCGAAAAGACGCGTCGGGGATTTTTG11.0869565217391304No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCCTGTCTCTTATACAC11.0869565217391304No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
GTCTTCTGCTTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA11.0869565217391304No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGTTGAA11.0869565217391304No Hit
ACACTTTTTTTTTCGTCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATT11.0869565217391304No Hit
GTGTAGGGAAAGAGTGTAGACTCGGGGTGCCGTCCCATGTACTCTGCGTT11.0869565217391304No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA11.0869565217391304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAAACCCCCCA11.0869565217391304No Hit
GTTGATACCACTGCCCCATGTACTCTGCGTTCTGTCTCTTATACACATCT11.0869565217391304No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATCTGCGTATAAATGTA11.0869565217391304No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACCTGTCTCTTATACACA11.0869565217391304No Hit
GCAGAGGCAGTGCGGATCCGGCGTTCTCCACTGATCTTTTCCAAGGCTGT11.0869565217391304No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAGAAAAAAAAAAAAA11.0869565217391304No Hit
CGTCAGATGTGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAGAG11.0869565217391304No Hit
ATCAACGCAGAGTACATGGGTGGCGACGGCAGCGAGGCAAAAAAAAAAAA11.0869565217391304No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTT11.0869565217391304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0869565217391304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGACC11.0869565217391304No Hit
GTTCGGAGATGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCTGA11.0869565217391304No Hit
CCTGTGCCGGCACGCGTCAAAATGCCGGCAACTGATAGCGGCCATACAAG11.0869565217391304No Hit
GATCTCGGTGGTCGCCGTCCCATGTACTCTGCGTTGATCTGTCTCTTATA11.0869565217391304No Hit
ATCTACACTCTTTCCCTACACGACGCTCTCCAAAAAAAAAAAAAAAAAAA11.0869565217391304Illumina Single End PCR Primer 1 (96% over 31bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATACCCCCCCA11.0869565217391304No Hit
GTTGGGGGCGCTGGGCCGGGGCAAGTGGCGCGAGAGCTCGGTCAGCGCCA11.0869565217391304No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAGCCTTGATATTAAAAAA11.0869565217391304No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAGATGCGTTTAAACCAC11.0869565217391304No Hit
GAGTACATGGGTGGCGACGGCAGCGAGGCAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit
GTATCAACGCAGAGTACATGGGGATACGGCGACCACCGCTGTCTCTTATA11.0869565217391304No Hit
ACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTGTCTCTTATA11.0869565217391304Illumina Single End PCR Primer 1 (95% over 22bp)
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAA11.0869565217391304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCCACGCTGCCCG11.0869565217391304No Hit
TCTCGGTGGTCGCCGTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT11.0869565217391304No Hit
CAAACAAACCATACACTAATAAAAAACTAACAGCAAACGAGACCCCGCAG11.0869565217391304No Hit
GCACGGCATGCCGTCGTCGCCCGACGAACTGGAGGCGCACCAGTCCATCG11.0869565217391304No Hit
CTACACTCTTTCCCTACACGACGCTCTTCCGAAAAAAAAAAAAAAAAAAA11.0869565217391304Illumina Single End PCR Primer 1 (96% over 32bp)
TGCAAGCGGCGAGCGACTCGCGGCGACCCCTCCCCGATCGCGGCGAAAGC11.0869565217391304No Hit
GGCGACGGCAGCGAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0869565217391304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)