FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003669792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAGGAGGCTGAGGCAGGAGAAGCACTTGA33.5294117647058822No Hit
TATCAACGCAGAGTACATGGGACGGCGACCACCGAAAAAAAAAAAAAAAA33.5294117647058822No Hit
GCCTGGAGCGCCGCCGCGTCCAGGTCGAGAAGCGCGCCAAGGGCGGCGAC22.3529411764705883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22.3529411764705883No Hit
AACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA22.3529411764705883No Hit
GGCACCCACAGCTTCCGCGGCAGGCCCTCAAGCCGATGCCAATGCCGCCA22.3529411764705883No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACTCAGAGTACATGGGTGGC22.3529411764705883Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTG11.1764705882352942No Hit
GTAGATCTCGGTGGTCGCCGTACCCATGTACTCTGCGTCTGTCTCTTATA11.1764705882352942Illumina Single End PCR Primer 1 (95% over 24bp)
GGTATCAACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATGC11.1764705882352942No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGTATCAACGCAAAAAAAAAAA11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA11.1764705882352942No Hit
GTGCTGGCCGCGGCCGGCTCGCTGTCGCTGTTCGCCGCGCGGCAGATCAT11.1764705882352942No Hit
TATCAACGCAGAGTACATGGGATGATACGGCGACCACCGAGACTGTCTCT11.1764705882352942Illumina Single End PCR Primer 1 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1764705882352942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGG11.1764705882352942No Hit
CTTTCCCTACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAA11.1764705882352942No Hit
TCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTGTCTCTT11.1764705882352942Illumina Single End PCR Primer 1 (95% over 22bp)
GGTGTAGGTGGTGGTCTTGTTGCGCGCGTCCTTTTGGCTCAGGCGGTTGC11.1764705882352942No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCGCGCGTCCGGCG11.1764705882352942No Hit
TCTCGGTGGTCGCCGTATCATCCCATGTACTCTGCGTTGATACCTGTCTC11.1764705882352942Illumina Single End PCR Primer 1 (95% over 21bp)
ACGCAGAGTACATGGGATGATACGGCGACCACCGAGATCCTGTCTCTTAT11.1764705882352942Illumina Single End PCR Primer 1 (95% over 24bp)
GTACATGGGAGATCGGAAGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACTGCGTT11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11.1764705882352942No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit
ATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAACTG11.1764705882352942No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT11.1764705882352942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAAAATGGGCCGCGG11.1764705882352942No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11.1764705882352942No Hit
GAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAG11.1764705882352942No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCGGTGTAAAAAC11.1764705882352942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCGCGCGGGCGGCC11.1764705882352942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.1764705882352942No Hit
TCGGAAGAGCGTCGTGTAGGGAAAGAGGTAGATCTCGGTGGTGCGTCCCA11.1764705882352942Illumina Single End PCR Primer 1 (96% over 27bp)
GATCTCGGGGTCGCCGTATCCCATGTACTCTGCGTTGATACCTGTCTCTT11.1764705882352942No Hit
CATCAGATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAGAGG11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAA11.1764705882352942No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAC11.1764705882352942No Hit
CATCAGATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACAGTGC11.1764705882352942No Hit
GGCCTACCGCCAGATCATCGGCCACCGCGACGTGGTCGGCAAGACGGTGC11.1764705882352942No Hit
ATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTGTCT11.1764705882352942Illumina Paired End PCR Primer 2 (96% over 30bp)
GGTATCAACGCAGAGTACATGGGACGGCGACCACCCCGATCTACGCCTGT11.1764705882352942No Hit
CCTGCGGGTCAGCCACTGACCGGCAGGTGCCTTGATGGCTTTTCAAGCTT11.1764705882352942No Hit
GTATCAACGCAGAGTACATGGGACGGCGACCCTGTCTCTTATACACATCT11.1764705882352942No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit
GCCCTGGGCCCTGAAGGTTCTTTTCTGGGCCATTCCACCGCCAACCCATG11.1764705882352942No Hit
GCCGACGAAGGCTTAGGGTAGATCTCGGTGGTCGCGCCCATGTACTATGA11.1764705882352942Illumina Single End PCR Primer 1 (95% over 21bp)
GGTGGGGGAAAAAAAAAAAAAAAAAAGTACTCTGCGTTCTGTCTCTTATA11.1764705882352942No Hit
GTAGATCTCGGTGGTCGCCGTCCCATGTACTCTGCGTCTGTCTCTTATAC11.1764705882352942Illumina Single End PCR Primer 1 (95% over 21bp)
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA11.1764705882352942No Hit
GATCTCGGTGGTCGCCGTCCCATGTACTCTGCGTTGATCTGTCTCTTATA11.1764705882352942No Hit
GTGGTAACAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATTTAAGAGGG11.1764705882352942No Hit
ATCAACGCAGAGTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAA11.1764705882352942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT11.1764705882352942No Hit
CATCAGATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATCTGC11.1764705882352942No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGGTG11.1764705882352942No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGTGCCGGC11.1764705882352942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT11.1764705882352942No Hit
GTGTAGGGAAAGAGTGTATTCGTGGCGAGCAAAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit
ATACACATCTGACGCTGCCGACGCCCATGTACTCTGCGTCTGTCTCTTAT11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT11.1764705882352942No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTT11.1764705882352942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGACCGGTTGGTAACA11.1764705882352942No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTGTTAATAA11.1764705882352942No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCGT11.1764705882352942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAC11.1764705882352942No Hit
GTACATGGGAATGGAGAGAAGGGAAGTCTGAAAGATAAGCAGAGGCTGGA11.1764705882352942No Hit
CTCCATCACCGTGCCCTACAAGGTGTGGGCCAAGTTCGGACACACCTTCT11.1764705882352942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG11.1764705882352942No Hit
GTGTAGATCTGGGTGGTCGCCCATGTACTCTGCGTTGACTGTCTCTTATA11.1764705882352942Illumina Single End PCR Primer 1 (95% over 21bp)
TATCAACGCAGAGTACATGGGGACGGCAGCGAGGCGAAAAAAAAAAAAAA11.1764705882352942No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAATAATCAGGGGGGGCGG11.1764705882352942No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAATATTACGCGCTGTA11.1764705882352942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)