FastQCFastQC Report
Tue 10 May 2022
EGAF00003669816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63719
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG5140.8066667712927071No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC3680.5775357428710431No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT3500.5492867119697421No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT3100.4865110877446287No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT3080.48337230653337315No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT2710.42530485412514324No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC2580.4049027762519814No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGT2570.40333338564635357No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC2460.38607008898444733No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA2450.3845006983788195No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA2380.37351496413942464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.3484047144493793No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT1990.3123087305199391No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG1910.29975360567491643No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC1900.2981842150692886No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACAT1850.2903372620411494No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT1710.26836579356235973No Hit
GTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAG1560.2448249344779422No Hit
GAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTT1550.24325554387231435No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT1420.22285346599915254No Hit
GGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTT1250.19617382570347933No Hit
CTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCT1150.18047991964720098No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA1130.17734113843594532No Hit
ACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCG1130.17734113843594532No Hit
AGTCAAAGCTGTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATA1100.17263296661906183No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT1090.171063576013434No Hit
ACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCA1020.16007784177403916No Hit
ATTTACATCTGAGAGCACAGCTGTCCTGGCATCAGGCACAGCAGCTGCAC1020.16007784177403916No Hit
GTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAAC1010.1585084511684113No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG1010.1585084511684113No Hit
GTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGG980.1538002793515278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.15223088874589996No Hit
GGTTTATATAGGTTGCTCTATTTACATCTGAGAGCACAGCTGTCCTGGCA950.1490921075346443No Hit
CCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCC950.1490921075346443No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG930.14595332632338864No Hit
CTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTG920.1443839357177608No Hit
GACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC920.1443839357177608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.14281454511213296No Hit
CCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCC910.14281454511213296No Hit
GGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCCCAGCA840.13182881087273812No Hit
GAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTG810.12712063905585463No Hit
GGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTT790.12398185784459895No Hit
TATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGC790.12398185784459895No Hit
CTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCA780.1224124672389711No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC750.1177042954220876No Hit
GTACATGGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGT750.1177042954220876No Hit
TATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACC750.1177042954220876No Hit
CCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTC730.11456551421083193No Hit
GAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCC720.1129961236052041No Hit
ACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGC720.1129961236052041No Hit
TGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTG710.11142673299957627No Hit
AACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTG710.11142673299957627No Hit
ATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCG690.10828795178832058No Hit
TTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTAC680.10671856118269277No Hit
GTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGG680.10671856118269277No Hit
CATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAG670.10514917057706492No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA660.10357977997143708No Hit
CCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTC660.10357977997143708No Hit
ATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCC650.10201038936580925No Hit
CCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCC640.10044099876018144No Hit
AAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGG640.10044099876018144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)