FastQCFastQC Report
Tue 10 May 2022
EGAF00003669820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132886
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6010.4522673569826769No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG5700.42893909064912783No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5030.3785199343798444No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA3900.2934846409704559No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG3610.27166142407778093No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT3560.2678988004755956No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT3560.2678988004755956No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG3430.25811597910991374No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG3350.25209578134641725No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA3290.24758063302379482No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA3100.23328266333549055No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT2990.22500489141068286No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA2720.20468672395888204No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT2720.20468672395888204No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA2580.19415137787276313No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.17533825986183646No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC2290.1723281609800882No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA2260.170070586818777No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA2240.16856553737790286No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT2230.1678130126574658No Hit
TACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGC2230.1678130126574658No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT2190.16480291377571754No Hit
ATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGG2160.16254533961440634No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT2040.15351504296916152No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT2030.15276251824872447No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT2010.15125746880785032No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC2000.15050494408741327No Hit
GATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATC1980.14899989464653915No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC1940.14598979576479088No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC1920.14448474632391675No Hit
GACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGG1910.14373222160347968No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA1800.13545444967867196No Hit
CTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCC1790.13470192495823488No Hit
TTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTG1780.1339494002377978No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT1760.13244435079692368No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG1740.13093930135604953No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA1740.13093930135604953No Hit
TCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCC1690.1271766777538642No Hit
GTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCT1660.12491910359255301No Hit
GAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGC1660.12491910359255301No Hit
CTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATT1620.12190900471080474No Hit
ATTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTTCAGAG1620.12190900471080474No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC1610.12115647999036767No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT1590.11965143054949354No Hit
AGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAG1580.11889890582905648No Hit
GTAGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTC1560.11739385638818234No Hit
TTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTTCAGAGC1560.11739385638818234No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG1560.11739385638818234No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA1560.11739385638818234No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA1510.11363123278599703No Hit
AGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCC1500.11287870806555997No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC1500.11287870806555997No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT1500.11287870806555997No Hit
CATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGT1450.10911608446337463No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG1430.1076110350225005No Hit
TCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCA1430.1076110350225005No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT1430.1076110350225005No Hit
CACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCT1400.1053534608611893No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCA1390.10460093614075222No Hit
CCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCA1390.10460093614075222No Hit
CCGTAGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACG1370.1030958866998781No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC1370.1030958866998781No Hit
GGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGA1350.10159083725900396No Hit
CCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTA1330.10008578781812982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)