FastQCFastQC Report
Tue 10 May 2022
EGAF00003669821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133825
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG5920.4423687651784046No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG5130.3833364468522324No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC4880.3646553334578741No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA3740.27946945637960025No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG3470.25929385391369325No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT3160.23612927330468897No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT3160.23612927330468897No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT3160.23612927330468897No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA3150.23538202876891462No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA2890.21595367083878197No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT2800.209228470016813No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA2800.209228470016813No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA2740.204745002802167No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG2710.202503269194844No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA2660.19876704651597238No Hit
TACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGC2340.17485522137119372No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT2330.17410797683541937No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.17037175415654773No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC2130.15916308611993274No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG2090.15617410797683542No Hit
ATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGG1970.14720717354754345No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT1950.14571268447599478No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT1870.13973472818980012No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG1870.13973472818980012No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT1810.1352512609751541No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA1800.1345040164393798No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC1790.13375677190360544No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA1760.13151503829628247No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC1750.13076779376050812No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA1750.13076779376050812No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT1740.1300205492247338No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC1670.12478983747431346No Hit
GACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGG1650.12329534840276479No Hit
ATTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTTCAGAG1620.12105361479544181No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC1620.12105361479544181No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT1580.11806463665234448No Hit
TCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCC1580.11806463665234448No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT1550.11582290304502148No Hit
CTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCC1540.11507565850924716No Hit
TTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTTCAGAGC1530.11432841397347282No Hit
TCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCA1530.11432841397347282No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG1510.11283392490192415No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA1510.11283392490192415No Hit
GATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATC1510.11283392490192415No Hit
GATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGT1490.11133943583037549No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC1480.11059219129460116No Hit
CTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATT1470.10984494675882683No Hit
AGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAG1460.10909770222305248No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA1450.10835045768727816No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCG1430.1068559686157295No Hit
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG1420.10610872407995516No Hit
TTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTG1390.10386699047263218No Hit
GTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCT1390.10386699047263218No Hit
CATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGT1390.10386699047263218No Hit
GAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGC1390.10386699047263218No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA1380.10311974593685783No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT1340.1001307677937605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)