FastQCFastQC Report
Tue 10 May 2022
EGAF00003669822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133825
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG5880.43937978703530733No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG5510.41173173921165696No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5280.39454511488884736No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA4140.3093592378105735No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG3460.2585466093779189No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT3320.24808518587707828No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT3250.24285447412665792No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG3180.2376237623762376No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG3080.2301513170184943No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA3070.22940407248272No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA2940.21968989351765367No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT2830.21147020362413602No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA2800.209228470016813No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA2720.20325051373061834No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA2660.19876704651597238No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT2550.1905473566224547No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT2490.1860638894078087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.1770969549785167No Hit
TACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGC2340.17485522137119372No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC2330.17410797683541937No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT2230.16663553147767607No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA2180.1628993087988044No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT1930.1442181954044461No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT1930.1442181954044461No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC1900.14197646179712312No Hit
ATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGG1890.1412292172613488No Hit
TCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCC1880.14048197272557444No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT1870.13973472818980012No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA1850.13824023911825145No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC1820.13599850551092846No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT1760.13151503829628247No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC1740.1300205492247338No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA1720.12852606015318513No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA1700.12703157108163646No Hit
TTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTG1690.12628432654586214No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC1690.12628432654586214No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG1680.1255370820100878No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT1630.12180085933121614No Hit
ATTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTTCAGAG1600.11955912572389316No Hit
CTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATT1580.11806463665234448No Hit
GATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATC1560.11657014758079583No Hit
AGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAG1540.11507565850924716No Hit
GAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGC1530.11432841397347282No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC1520.1135811694376985No Hit
GACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGG1510.11283392490192415No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT1510.11283392490192415No Hit
GTAGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTC1480.11059219129460116No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT1480.11059219129460116No Hit
CACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCT1460.10909770222305248No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA1440.10760321315150383No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG1430.1068559686157295No Hit
TTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTTCAGAGC1430.1068559686157295No Hit
TCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCA1410.10536147954418083No Hit
CATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGT1400.1046142350084065No Hit
CTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCC1400.1046142350084065No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1380.10311974593685783No Hit
GTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCT1380.10311974593685783No Hit
AGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCC1360.10162525686530917No Hit
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG1350.10087801232953485No Hit
CAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAA1340.1001307677937605No Hit
CTCCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGT1340.1001307677937605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)