FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003669961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length100
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTCATCCTCTGCCGCACGCATCGCCGCTTCCCGGCGTGCCTCTAGCCA64.37956204379562No Hit
GCGGTACACGCCGGGCTTGAGCGGCAAGGTCTGGAGCACGTTGCGGATCG42.9197080291970803No Hit
TCCTACCGGAGGGGTCACGGCGGCCCTAGCGTTCTGCCATGACCGACCTG42.9197080291970803No Hit
GGATTCAATGTGAGGAGAAGCCCAAACACCTTGTCTTGCTCTCTCCTCTT32.18978102189781No Hit
CCGATGATCATGATGGTGACTTTACGGCCAAACGTCCTCGTCCACGACAC32.18978102189781No Hit
TCACAACACCCTCGTGGGCGCGGGCTTGCGCGACCAGATCAAAATTGGCG32.18978102189781No Hit
ACGGACAGCCGATGACCGGGCAGCGCGCCGCGGCGCGCGACTCGCTGCGG32.18978102189781No Hit
AGTGATGGCGAAGGGCAGCGTGCGCAGCGCCTCTTCCAACAACGTGCGCT32.18978102189781No Hit
GAGTGGTAAAGATCTCGATGGCCTGCGAGACTTCGGATGTGCTCGATGGT32.18978102189781No Hit
TTGTCTTCGAAGGCATGGGCACTGGGGCCCGCCAGACCGAGAAGACCCGC21.4598540145985401No Hit
CTCCTCGACGACGCCCGAACCCTCGACGAGCTGCGCACCTGGCTCACCCA21.4598540145985401No Hit
ATGTTATTTTTTGACGCGTTCGGCACCTTACGAGAAATCAAAGTGCTTGG21.4598540145985401No Hit
GTCGGCAGCCCGGCGATGAGGCTGCGCCGCAGCGCCTGGATGTTGTAGCG21.4598540145985401No Hit
ATTTAAGGGCATTGGTGATCCGAGTTTTATGTATGTCATATACCAAATGA21.4598540145985401No Hit
GGGGACGGGCGAGGCAGGGGTTGCCGCGACGACGGAGCGCTACGAGGCCG21.4598540145985401No Hit
GTCGCACGCACCCTGGCCGATCTGGAGCAGGCCGAACGCATCGGCCGGCA21.4598540145985401No Hit
ACTTATACGTTCACGAGCTGCCTCCTTTCCGTGACCTCACTAGAGAGGTC21.4598540145985401No Hit
GGGAGTATTTGCATGTACCCAGTGTCATTAGTAGCAGAATACTTGGGATT21.4598540145985401No Hit
CGTCGGGAGGAGACACTCCGACGATGGCTGGAGAGCCTCTCGCGGGAGCA21.4598540145985401No Hit
GAGCCGATGGGCATTGTGGACGAGGCGATCGAGCACGGCGTCGGCATAGG21.4598540145985401No Hit
ACTTTCATGTACACGTGATTCCCTGGGCATCTTGTGGAAATACAGATTCT21.4598540145985401No Hit
CGCAGGAGCCGCACGTCCTGCCGGCCCGGTTCCCGAACCTGCTGGTCAAC21.4598540145985401No Hit
AAGGTATCCTGCCCGCTACTTGACTGCGACGGTGCTTCGGATGTTCCAAC21.4598540145985401No Hit
CTTTGCCTCAACACAGCCTTTGCAGAAGTGGGCAACCGATATAACAGAAT21.4598540145985401No Hit
CCGCTCCCACGGGTCCGAAGGCTCCATGCGCGCAGCGGTCGACTGCACCC21.4598540145985401No Hit
TCCAGCAGCTGTTCGCCGAGCTCGCCGTGACCCTGGTGCCGCAGTCGGCG21.4598540145985401No Hit
GCCTCCTCCAGACGCTGCTGGTAGGCGCGCAGCTCGTCCTTCGTGGGCCA21.4598540145985401No Hit
GTTCGGTCGCGGTGCACAAGATGGCGATGAACCCCGACATCTTTCCCGAC21.4598540145985401No Hit
GTAGTATACTGTCACTTCTGGATTGATAAGCTGAGGAAGAAAGTTAAGTT21.4598540145985401No Hit
GTATGAATTAATCCCGGTCAACAAAAACCTGGTGTTGGTATTTTTTTTTG21.4598540145985401No Hit
GGCGACCGTCGCCGCGCGCGAGAGCGAGGTCATCGAACAGCCGCGGACCA10.7299270072992701No Hit
ACCGTCGCCACTTTGACCGAAGGAAATCGCCTAAGACGCAGTTTGTGACC10.7299270072992701No Hit
CCCCATCCCTGGGAAAGATCCGACCTCGGATCATGATCCTCATCACCATG10.7299270072992701No Hit
CTGTTGCGCATGCTACAGGTACTTATTGTAGTCCTCTGCTTCACATTTCT10.7299270072992701No Hit
GTCGACATCCCGCTGGGCACGCTCACCTGCGTTACCGGCGTTTCCGGCGG10.7299270072992701No Hit
GGCCGGTCACTGTCAATGCAGTGACCGGCCCGACGTGTCGTCGTGGCGAT10.7299270072992701No Hit
CCACCACCACCACCACCGGAGGCAACAACTCCACCAGCGGCAACAACCCC10.7299270072992701No Hit
ATGCGGACGTGCAGCTCGTCAAGGAGGCGATGTGGGATCCCTTCGAGTAC10.7299270072992701No Hit
ATCCCAGCCCGGCCTGGGGTTGGAATTCCGGCCGTTCGCCAAAGAAGAAC10.7299270072992701No Hit
GCACTACGACGTGCAGCCTCCGGAACCGCTGGAGCTCTGGGAGAGCGACC10.7299270072992701No Hit
GCCCGTACGTTCTCACCAAATGGGGCACATGGCAGGCTCCTCTCTCGCCC10.7299270072992701No Hit
CTTGGTGGCAGGAAGGAGAGGTGCCAAGCAAAAGGGGGAAAAGCCCCTTA10.7299270072992701No Hit
GCTGCAGCTCGCCGCCACCATCGGGCGCCAGTTCGATGCCGAGCTGCTGT10.7299270072992701No Hit
CCGGCGGACCGAGCTGGAAGGTCACCCGTGGAGCTTGCTGCGGGGTGGAC10.7299270072992701No Hit
GAACACGCTCGTGCCATCCACCCGCCGGAACGCGACGGGTGAGGTGGCGA10.7299270072992701No Hit
CCCCTGGATTCTAAGCAATTCTCCTGCATCAGCCTCCCGAGTAGCTGGGA10.7299270072992701No Hit
GGCTTATTTTTCTACCCTAGGGAAAGGAAGCACACCAGACATATGTAACC10.7299270072992701No Hit
CTGATAAAAGTGTTTCTGCCGCTCTGCCTGCCCGCCATTGCCGTCACGGC10.7299270072992701No Hit
ACCCTGGACCGCACCGTGCACTGTCAGCCCGCGATCTTCGTGGCATCGCT10.7299270072992701No Hit
GGAGACGGCAAGAGTATCCACGCTGCCCAACAGAGCACCCAACGTGCTCA10.7299270072992701No Hit
GCCTGCCGGAGGGGACCACCTTCTCTAGAGGCAAGGGCATGGCAAGCGGC10.7299270072992701No Hit
GGCGCATGGAGGCGGTGATGAGCCAAATACATACAACTACACTTCGTAAT10.7299270072992701No Hit
CTTCAGGCTTGCCAGATACCCGATCGGGGCGTCGAGCCAGACATAAAAGT10.7299270072992701No Hit
CTTATACCTTATCTGATTCCTCTTCACTCATGCAGCTTCATCTCAACAAT10.7299270072992701No Hit
CTCCTCATTCATTCAATGTTTATCATGAAATTGCAGCAATTCAGTTACCT10.7299270072992701No Hit
CCATCAGGCCGATGGCTCCGATGGCGGCAACGAACCCGCATGCCGTGATG10.7299270072992701No Hit
GTCATGGCTGGTATGATATACGTCTCGAGCACGTGCTTCTCATCGCCGTA10.7299270072992701No Hit
GTCGACCATCGCCACGCCGGGGTAGAGCAGCAGCCGCCCGCCCGGCGCGA10.7299270072992701No Hit
CTGCCTCCCAGGCTGGAGTGCAGTGGTGCGATGTTGGCTCACTGCTGCAA10.7299270072992701No Hit
GCCTTTGAGTCCCATAAGAAGCCAGGATCTGTGGCTTCTGATTTCCTCAT10.7299270072992701No Hit
GAACTCGCTCGTGGGGGAAGCACCCATGGGCATCATGGGCGCACCTCGCG10.7299270072992701No Hit
GAGCAAGAGTCCAGGGAATACTAAAATAAAGTCCTGACGGTGGGCAAGTG10.7299270072992701No Hit
TCCCAACGGGGAGGGCCTACGTGGAGCCGAGTCTCGCCGTCGTTTCCGCG10.7299270072992701No Hit
ATCATGGACCGCCTGGATTCACTCCTGCCGTCGGCATTTGTCACCTGGCT10.7299270072992701No Hit
CTAGTTTAAATTGTATTTCATGCTCGAACCAACAGCCTAGCCCCAATCTG10.7299270072992701No Hit
ATTCAGCAGTGACTAAAATAGAAACGGGTCTACCTCAGGAAGACCATTAT10.7299270072992701No Hit
ATCCGCGACGGGACGACGAGCGCGTCGAGGTCGCGGGTGTCGGCGACGAG10.7299270072992701No Hit
CCTGCAGGCCCGAGCTTTCGCCGGCCTGGAGCTGCAGCAGCTTGTCGGGA10.7299270072992701No Hit
GTGGCGGCGCATCGAGTTCTACGTCGCGCAGGCGCGTGCGCTGGAAGACT10.7299270072992701No Hit
TCATGCGCGGTGCCACCGGAAATAATTGTTTCGTTGATATACGGATAGAT10.7299270072992701No Hit
GATAAGGGGCGCCTATTGCACTTTGTGACAGCAGCCACAGAAACTGATAC10.7299270072992701No Hit
GTAAAATTCCTAACAGCGACTGACTGAGGCAGGAACATCATAGTAAGGAA10.7299270072992701No Hit
ATTTGGGCGAGCTCGGCGAGGTCACCTTGATGTCGCCGCTGACGATCTTC10.7299270072992701No Hit
CTCACGGCTTTGGCCGAGCACGAGCTTGCCGAGCGCGACCGGCGTCGCAT10.7299270072992701No Hit
GCTCGATGTTCTCCACCGTCTCGGCGAAGCCGCCGGCCGTCTCGCCCGGT10.7299270072992701No Hit
GCCTCAGGTTCTTGAGGACCACGGCTTCAACAAAAGGTGTTAATGGCTGT10.7299270072992701No Hit
CTACGCCGGCGGCCGGTACGCCATCGAGTCGTACCTAGACCACCACGCGA10.7299270072992701No Hit
TGCCTGTGGCGGCGGCCCGGGATGAGCCCCGTCTCTCAGTTGTCTCTGAG10.7299270072992701No Hit
GGAAGTACTTCTTCTCCAAAGGAATACAAATGCTCTTTGTGGATGACATC10.7299270072992701No Hit
CCGCTGAAGACCATCACCGAACGCTGGCACCTGCCCGGCGACTGAAGGGA10.7299270072992701No Hit
ATTGCAGCAGCAGCGCCTTCTGCATCGGCGGCGACATCTGGCTCGGCGCG10.7299270072992701No Hit
CAGGGCAGGTCTGAAACAGTGACTTACTTGTTAATAAACTGGCATTTTCC10.7299270072992701No Hit
ATGTCCCCAGCGCGGATAAAGAACCTTGGTTGGGTGCAGTAAGGCCGCAG10.7299270072992701No Hit
CCGCAGCTCCTGGGGCGTTAGCTCAGCCGGTTAGAGCAGCGGACTCATAA10.7299270072992701No Hit
GTAAGGTCCCGCTCAACGAGATCACTTCTCAGGCAACCTATAAAAGTGGC10.7299270072992701No Hit
GGGTTGGTCACCGCGATGAGCACGCCGCCCACGATCAGGGCGAGCACGAC10.7299270072992701No Hit
GGATCTCCGACCCGCGCGTCCTGGAGGCGATGGGAACCGTCCCGCGGGAG10.7299270072992701No Hit
CCCGTGAAGCGTTCCGGCTTGCCTCTGCGAAGCTTCCGATCGACACCGTG10.7299270072992701No Hit
ACACGTGCCGTCCTGCTGCACGTTCTCAGCAGCACAACCGACCACGTAGA10.7299270072992701No Hit
CGACCACGCCCCGGCGCAGGCACGCCAGCGCGTGCCGCTCCACCCGCTTC10.7299270072992701No Hit
GTGGGGTTCATCGGGCTTGCCGAGACGCTTACGGCGTTGATCGGAGTCCA10.7299270072992701No Hit
CATCGAGTCTCAACATCCGCACGTCCAGAGTACACCCACGGTGATAGAGC10.7299270072992701No Hit
ATCCACCACAGCGACGACAGGATTTGACCGTCCGTGTAGTTAGGCCTACG10.7299270072992701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)