FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003669962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003669962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length100
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACAGGGACGCGACCAGAAGCATCCATAGGAGACGTAGTGTCGTCGTCG64.37956204379562No Hit
GACCAGGTGGCCCCGGTCGAGGTCGTTGTGGGCGTAGACCGGCGGGCCGG42.9197080291970803No Hit
CTCCTACATGGCGGGCATGTCCGGTTACGGCCCCCCCGACCGCTTCAACG42.9197080291970803No Hit
CCACTGGTTATGGATAACCCGGATTCCCAGAACCGTATCAGGACCAGTGG32.18978102189781No Hit
GGGCCACAGTGCTCGATGCTTGCTACCCGGATGCCCAACACGACTTTCCC32.18978102189781No Hit
GATGAAGGCAAGGGCCGCCCATGGGCCGCACTCGGCTGCGAGGTGGCCAT32.18978102189781No Hit
TTCCGATGGAAGCTGTGCACCCGCTCGGCCTTGTCGCTGACCACGAGCGC32.18978102189781No Hit
CGGCTTGGCCCCGGCACGGTGCGCGATGCCGAGCCTTTCGAGCAGCCCGT32.18978102189781No Hit
CATCTATTCTCTTCTTTGTCATCATACTCATCTAGTTTATTGCTTCATCT32.18978102189781No Hit
GTGGGAGGCGATCGACGAGGCCGAGATGTCGGAGGCCGCGCGGCTGGCGC21.4598540145985401No Hit
GGGCTATTTAGCAGGTTAAGGTCTCGTTCGTTATCGCAATTAAGCAGACA21.4598540145985401No Hit
GCTCGGGGTCGTCGTGGCTGCGCAGCGCGACCAGCGTGCTGACGAGCGCG21.4598540145985401No Hit
GGTGGGAAGACGAGGGCTGTTTTGCCTCCATGCTGCGCTCCTACGGTTGT21.4598540145985401No Hit
CCGAGATCCCTTCCGTGATTTGAGGGGTTTGTAACAGCTCCCCTTTGACC21.4598540145985401No Hit
CATGTGGGCTACCTGTTCGAAATTGGGGGATTCAGACAGGTTATAGCTGA21.4598540145985401No Hit
GGGCTCGAGCCGCTCGACGCTGGTGCCCGTCACGACCTCCTGGAAATCCT21.4598540145985401No Hit
AACCAACGGCGACCACGCCCGACCGCGCGCAGCTCGGGCCCCGCGCGGAA21.4598540145985401No Hit
CCGGACGCATGCCGATCACGAGGCCGGAGGACAGCAGCAGCTCGTGGGTC21.4598540145985401No Hit
TAAAATTGGTGCAACAAAACATTGTTGTAATGTTTCAATGCCAGTTTAGA21.4598540145985401No Hit
ACCCTCACGCCCGCCGAGCGCCGCCGTGCCGAGGCCGCGCTGGTGGTCGG21.4598540145985401No Hit
GGTTTACCCATCCCAAGGACAAAAAATATCGGGACAGAGCAGTGGGTGTC21.4598540145985401No Hit
CCTCTTGCACGGCGCCGGCCAGCCCCGTTCCCTCGTTGATGACGGCCACC21.4598540145985401No Hit
GACCACTAAGGATCCCGGAGAGGAACTTCAGGGAAACTAAACACAGCAGC21.4598540145985401No Hit
GACTTGAGCTAATGTTTAGTTTCAAGTCAACAAATAGCATTTCATTTCCT21.4598540145985401No Hit
GGTCAATCAAGATCTCAAAATATACCACACTGAAGTTCTGTCAGGCCAAA21.4598540145985401No Hit
GCGCGGAGCTGACCGTGTACGAGAACGTGAACCGCGACGGTGAGGTCGTC21.4598540145985401No Hit
ACATAGTGCTGCTGCTATACTCCTAATATCAGAGATCGTATCCATAAGCT21.4598540145985401No Hit
GCCGCTCAGGGCGTCGAGCACCTCTCCGGCTCGAGAGGGGGTGGCGGCTG21.4598540145985401No Hit
TGATAGGGCAGCGCGAGGTTGACGACATGCGTCGCACCAATCTCGCGGAT21.4598540145985401No Hit
CCCGCGATCAACGCGCCCGTGGGGAAGTCCGGCCCCTTGACGATCTTGAT21.4598540145985401No Hit
TTCCAATCCCAGCAAGCAATCCCTGGGCCTACGATGTGAGGTGAGAGATG10.7299270072992701No Hit
ACTGCACTCTAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAA10.7299270072992701No Hit
TATCAACCCTGATGCAAACAGGGGTTTGGCCGGCGGGCTGCCGGCGGAAA10.7299270072992701No Hit
CCCATCCGACCCTCGACCGCCTCAACGCCCTCGGCCTCCACGGCATGGCC10.7299270072992701No Hit
TTGCTGCCTCCTTCAGGGGCATGCTGAATGGCAGCGACAATTCATCTTCC10.7299270072992701No Hit
CAGTAAGAACGGTGCATTCCTTCTTGCTACTTCCTTCTTGCTACTTCCTT10.7299270072992701No Hit
AAACCGAAGCCATGCGCATCCAAATCGAAGTCGAAGTCGATGGCCTGGAC10.7299270072992701No Hit
AGCCAGATGAAAAGGATGGCGACCGGCAGTGTTGCAAGAAAGGTGGCTGC10.7299270072992701No Hit
GCGTGAGGCGCTGCCCGCGCTTGCGTCCACCGGCGTCGAAGGATTTGCCG10.7299270072992701No Hit
GATCCATTCGGACGAACCGCCCCGACAGTCCCTCAGCCGGGGTGGCCAGG10.7299270072992701No Hit
GCAGACCGTCGTCGCGCAGTGCCAGCCCGAGAACCAGAACAACGCGCTGA10.7299270072992701No Hit
TCTGCGGTCCGACGCCGAACCGCATCCACGACGAGGGCGGCACGGGAACG10.7299270072992701No Hit
GCCTGCGGCTCGGTTGCGACGACGAATACGTGTACATTACGGTATGGGAC10.7299270072992701No Hit
TAGAAGGGCGGCAATAGAGTCGAGTTGTTCACCCATATATTCCTCCTCTT10.7299270072992701No Hit
GGTGGCCAACGGGCTCATGGCGTTGCGCGAGGTGACCCGCAAGGCGGTCA10.7299270072992701No Hit
GGCTTGTAGCCGCGCTCGTTGGACTCCGGCAAGCCGGCGAACCAGTCGCG10.7299270072992701No Hit
CCTGAGGTCAGGAGTTCAAGACCAGCCTGGTCAACATGGTGAAACCTGTC10.7299270072992701No Hit
CGGTGGCCGCCTCCAAGGAGGCCAACATCCCGGTGCTGGCGCGCTACGCG10.7299270072992701No Hit
GTGAAATTTCTGATCAAAAGTAGTTTGGTGATATGGTTGGCTATGTACCC10.7299270072992701No Hit
CGGCAGAACTTTAGAGCAACCACATTCCATCATGTTGATCAGTAATTCAT10.7299270072992701No Hit
CATATCAACATTAACAGGAGTTTGGAAGAAACTGGTTCCAATCTTCACGG10.7299270072992701No Hit
CCGTTGGTGTCCTAGGGCGGATTATGTCCCGTCAGCCCCGTCGTCAGTGT10.7299270072992701No Hit
TCCGTCCACAACAACCATTCCCATGGTGATGAGGATCATGATCCGAGGTC10.7299270072992701No Hit
GATGTGTGCCCCGCTGCTGTCGACGCGGGTACAGCAGAATGTGCGCGCCC10.7299270072992701No Hit
GTGCCTGACCGCCTGCGTCTTTTTTTTTTTTTTTTTGAGTTAGAGTTTCA10.7299270072992701No Hit
ACGGCCTTGAACTTCTTGCGCAGGCGCCCGGGCGGGACGGGCTTGCCCGG10.7299270072992701No Hit
GGCTGCTCGCCGCGCCGGAGCTTGTCGGCCACCTTCTTGGACAGGGCCAC10.7299270072992701No Hit
GTATAGGCCGGGTGCGGTGGCTCATGCCTGTAATCCCAGCACGTTGGGAG10.7299270072992701No Hit
GTGCCGTCGTTGAACACCGCGCAGTGGTCGGCCGTGACCGAGCGCGCCAG10.7299270072992701No Hit
GCCAAACATTGCGCTGCTCGGCATCTTCTTTGGAACGTTCACCGTCAGCA10.7299270072992701No Hit
CTCTTCTCGTGCTGTGCGCGATCGGCGGGCTGTGGAATTACCCCATCTTC10.7299270072992701No Hit
ATCGTGTGCACCTCGCCGGCGAGCTCGGCGAGCACCGCCGCCTGGTAGCC10.7299270072992701No Hit
CCTCGGGATCCAAAATCTGCCATGTCCATAGATTTGTTATAGCCTGCCAA10.7299270072992701No Hit
GCACGAGGTCGGAGGTGCGGGTGAACAGCACTCGCCAGCCCTTCTCAAGC10.7299270072992701No Hit
AAGATGTACTTTCCGATCCTCGTCGTCCCGGTATCGTCCATCTCGACCGC10.7299270072992701No Hit
CCCAGAGGCAGGCGTCGGCGGAGAGCAACTCCTTGTTGGCGCGGACGAAG10.7299270072992701No Hit
CAAGTACCGCCTGCGCTACCCCGACGCGGCCGCGCGCCGCGCGCCCTTCG10.7299270072992701No Hit
TAGCTGATCTGCGCCTCGGGCAGCGCCAGCGCCACGCCCTTGGCGTAGGC10.7299270072992701No Hit
CTCGGCCCGCAGCGGCGCGGCGGCGCGGCCGAACAGCAGCCAGCCCGCGG10.7299270072992701No Hit
TTCCAATCTCAAAGTAAATAAGAAGTAGCGAGAAAAAGTGGATTCATGCA10.7299270072992701No Hit
ATCATCATGATTCTTATCAAACCAGGATGACCCAATTGTTGAGATGAAGC10.7299270072992701No Hit
GTGCTGGGCGTAGGAAATCGTTTCGTCGTTGAGCGAGCGGTGCTGCGTGA10.7299270072992701No Hit
TGTGAAAACATAGACTGTTGGGCACACTATCCCCAGAATTTCTGATTTGG10.7299270072992701No Hit
NTGTGGAAGAGCGTTTCATTCGTGGAAGGCTGTGTTTGGACATCTCAGGT10.7299270072992701No Hit
TCATGGCCCATGCCTGCCTGGAGTCGATCTGCCTTATCGCCGATTCGTGC10.7299270072992701No Hit
CCGCCTGCACGTCGTCGACCGCGAACGAGGTGAACGGGATGCCGTCGGCC10.7299270072992701No Hit
ACTCGGGCAGACCGTAGTCGGGGTGGTGGGTGAGAAGCGGTCCACGTGGC10.7299270072992701No Hit
ACAGCGCGCTGCTCGTCGAGCTCGGCGACAACCAGCTCGACCTGCGCCAG10.7299270072992701No Hit
GGCAGACCCCAACCAACCAATTGCCATTTGTGGCCATCGTCCGGTACTGC10.7299270072992701No Hit
GTCATGCACCTGACGTCATACTTTTAGTGCTTGGCAGGAGTGGTTGAGAA10.7299270072992701No Hit
TGGTGGAGCCGTTCGGCTTTCTGATCCGCATTATTGAGTGGTGGCAGACC10.7299270072992701No Hit
CTACATCAAGGGCCAGTGCCCGCGCTGCGACGCGCCGGATCAGTACGGCG10.7299270072992701No Hit
AACTCCGGTAGATGGAACCCCGCCCCACAGCCGACATCGACGACATCGCG10.7299270072992701No Hit
AGCAGAAGGTGGTCATCGCCCGCGAGCTCAGCCGCAGCCTCAAGCTGTTC10.7299270072992701No Hit
CTCTCCTGTTACGCTGAGGTGCTGCGCCACCGGCGGTGATAATAACATGT10.7299270072992701No Hit
GTGTCGACTCCGACCTGGCCGTCGCCGACCCGTCGGTGGCCAACCAGATC10.7299270072992701No Hit
CGCCACGGTCACTCTGGTAGGTGGGCAGGTTAGCGAGATCAACGAGTATC10.7299270072992701No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGCGGGGGGGGG10.7299270072992701No Hit
ATGCAGACAAACAGCGGCCCAACAGCGGATCCGAGGACGTCGATGCGGAT10.7299270072992701No Hit
GCCAGCTCCTCCTGCTCGGCCACCGGGTACAGCCGGTCCGAGGTGACACC10.7299270072992701No Hit
AGCCTGCCCTGTGGAGGAAATGCCAGTTTATTAAATAACGAGTAAGTCAC10.7299270072992701No Hit
AAACTGAGCAGCTTAAACAGCAGAAATTTATTGCCTTTCAATTCTGGAGG10.7299270072992701No Hit
TGACTGACCTGCCTTTTCACGCAGCACGGTGCGCACCCGTGCGATGTCAC10.7299270072992701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)