FastQCFastQC Report
Tue 10 May 2022
EGAF00003670239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8564
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1021.1910322279308736No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT650.7589911256422233No Hit
GTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGAC320.37365716954694067No Hit
GATTAGATGTGGGCGCTACCAAATGTGAAGACAAGACAAGACTCTCAGGT320.37365716954694067No Hit
ACCATGTATCGTGCTCTTGCTAAAAGGCAGGCACAGTGATAGGCATTTGA320.37365716954694067No Hit
GGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAAAGCATCC290.338626809901915No Hit
GTGATAGGCATTTGAAATATCTCATGCTGTCTATCTAACTCACAACAATT230.2685660906118636No Hit
ATAGTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAA230.2685660906118636No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG220.25688930406352173No Hit
GTCGTATAATATTTGACTTTCTCTCTTGTTTTACAGGCAATCTGTGAGAA220.25688930406352173No Hit
TCTTAAGACAGGTTCTATGAGCACATGGTTTTGAGGGCTCAGCAAATCCC220.25688930406352173No Hit
CCTTAGAGGCTTATCATCCCCACAGGAGGACACCTGCCTAGTGGAGCTTT220.25688930406352173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.24521251751517983No Hit
TTATACGACTGCTTAATAATTCATATGCGCTACCCGGGAATCAATTCATA190.22185894441849605No Hit
TTATTAAGCAGTCGTATAATATTTGACTTTCTCTCTTGTTTTACAGGCAA180.21018215787015412No Hit
ATCTCATGCTGTCTATCTAACTCACAACAATTCCATAGGTAAGTACTTCC180.21018215787015412No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT180.21018215787015412No Hit
GCTACTAAGCGGTAGAGTTGGGATTTGAACCTGCATTGTTCCAAAACGTT180.21018215787015412No Hit
ATTTCATACAGCTACTAAGCGGTAGAGTTGGGATTTGAACCTGCATTGTT160.18682858477347034No Hit
GTAGAGAGGGGGTTCCACCATGTTGCCCAGGCTGGTCTTGAACTTCTAAC160.18682858477347034No Hit
GTACATGGGGTGGTGCGCTCGCCGCTGAAGGTCGACGTGGTGCGCGACCG150.17515179822512844No Hit
TAAATAAGCAGTGAGACCACTATTTTTTCTACTTCCAAAACAAACCCTGA150.17515179822512844No Hit
CTTACCAGCTGACCACTCTTTAGAACTTTGCTGTTGATCTTACATAAGCT140.16347501167678655No Hit
GTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAAAGCATCCAAGAT140.16347501167678655No Hit
CATCTAATCAGTCAACGTATACTATCGACTCTAATTCCATTATCAGTCAA140.16347501167678655No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT140.16347501167678655No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC130.15179822512844465No Hit
TCCTAGAGGAGTCAAATTCATAGAAAGGAGAATCGTGGTGGGCATGGCTG130.15179822512844465No Hit
GGTCTAACTCATGATAGGTGCCCACTCAATGCTGGTTCTGCCACTCAGCA130.15179822512844465No Hit
GGGTTTCACCACGTTGGCCAGGTTGGTCTCGAACTCCTGACCTCAAGTGA130.15179822512844465No Hit
ACGTATACTATCGACTCTAATTCCATTATCAGTCAAAAGTGTCCCCTCCT130.15179822512844465No Hit
GCCTAGGCTGGAGTGCAGTGGTGCGGTCTTGGCTCACTGCAAGCTCCGCC130.15179822512844465No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT130.15179822512844465No Hit
AGTCAGGATGGTCTTGATCTCCTGACCTCATGATCCTCCCACTTTGGCCT120.14012143858010276No Hit
GTTATGACCTGCTTCTATCCCAATCAGTTTCTCCTGCCCCGTTTCGAGCA120.14012143858010276No Hit
AGTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGA120.14012143858010276No Hit
CCACCATGTTGCCCAGGCTGGTCTTGAACTTCTAACCTCAGGTGATCCAC120.14012143858010276No Hit
CCTTTAGACACAGGTCCAGGGCCAGTGAAAGCTCCACTAGGCAGGTGTCC120.14012143858010276No Hit
GTACAGGTCTAACTCATGATAGGTGCCCACTCAATGCTGGTTCTGCCACT120.14012143858010276No Hit
CATATAGACAAATCCCTGGAAATTCAACAGAGAACAATCCAAAAGATTTG120.14012143858010276No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT120.14012143858010276No Hit
ACCTGAGACAGGGTAACTCATAAAGGAAAGAGGTTTAATGGACTGTCAAT120.14012143858010276No Hit
CCATAGTACAGGTCTAACTCATGATAGGTGCCCACTCAATGCTGGTTCTG120.14012143858010276No Hit
CATCTAAATAAGCAGTGAGACCACTATTTTTTCTACTTCCAAAACAAACC110.12844465203176086No Hit
GGCTGGAGTGCAGTGGCGTGATCTTGGCTCACCGCAGCCTCCACCTCCTG110.12844465203176086No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT110.12844465203176086No Hit
GTATCAACGCAGAGTACATGGGCCAGCAGAAGGTCGCGCACCGCGCCGCC110.12844465203176086No Hit
TCATAGGTGTGTGCCACCACACCAAGCTAATTTTTTATTTCTATTTTTAG110.12844465203176086No Hit
GGATAAAGGTGAAAGCATTTTCATAGTTGAAGCAAATCTTAAGACAGGTT110.12844465203176086No Hit
GAAACAAAGCATCCAAGATGTGAAATAATTTGCCAATTTCATACAGCTAC110.12844465203176086No Hit
ACCAAATGTGAAGACAAGACAAGACTCTCAGGTTTCTGGGTAGGAAGTCC110.12844465203176086No Hit
TCCTAGGTTCTTTGACCTTAGGGGGTTCCCTCAAGTCAGTACCCAGTGTT110.12844465203176086No Hit
ACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAAAGCATCCAAGATGT110.12844465203176086No Hit
GGCTAAGATAATGTAGTTACCATGTATCGTGCTCTTGCTAAAAGGCAGGC110.12844465203176086No Hit
CCTGTAGTCTGCTGGTAAAGGATCCAAACAGGAGGGGACACTTTTGACTG110.12844465203176086No Hit
GTCTAAAGGTGGGCATTAGCAGTCGGCATCTCACTGCTATCACTGTGGAT100.11676786548341896No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT100.11676786548341896No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG100.11676786548341896No Hit
AGTCAAAAGTGTCCCCTCCTGTTTGGATCCTTTACCAGCAGACTACAGGA100.11676786548341896No Hit
GTAGTTACCATGTATCGTGCTCTTGCTAAAAGGCAGGCACAGTGATAGGC100.11676786548341896No Hit
GTCTCGTTCTGTCTCCCAGGCTGTAGTGCAGTGGCGTGACCTCAGCTCAC90.10509107893507706No Hit
GGATAGAAGCAGGTCATAACAGGAACTGCAGCTGTAGTTGGAGAGAGGAA90.10509107893507706No Hit
GTGGATGGTGATCGGCGGCCCGGTGGCCGTGATGATCGCGAGCTTCGTGA90.10509107893507706No Hit
GGCTGTAGTGCAGTGGCGTGACCTCAGCTCACTGCAACCTCCGCCTCCTG90.10509107893507706No Hit
TTCCTAACGCCCTCATGGTGGTGGTGATTTCTTTCCATTCCTGTAGTCTG90.10509107893507706No Hit
CCCTCATGGTGGTGGTGATTTCTTTCCATTCCTGTAGTCTGCTGGTAAAG90.10509107893507706No Hit
TTCCATAGGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAA90.10509107893507706No Hit
GCTAAAAGGCAGGCACAGTGATAGGCATTTGAAATATCTCATGCTGTCTA90.10509107893507706No Hit
ACCTGTGACTATCCAAGGCTGGGCACGGTGGCTCATGCCTGTAATACCAG90.10509107893507706No Hit
GCTGGTAAGTATGCCATGATCATTCAGATGCCTCATTTCCTCCTGGGCGT90.10509107893507706No Hit
TACATACACCTTAGAGGCTTATCATCCCCACAGGAGGACACCTGCCTAGT90.10509107893507706No Hit
GTCAGAGACAGTGGGGATTAAAACAGTCCCTTAACGGGTATGAGGTTAGA90.10509107893507706No Hit
CCACCACACTGACTAATTTTTTATTTTTAGTAGAGATGGGGTTTCACCAC90.10509107893507706No Hit
ATGCTGAACTGCATGAGGGCCTCTTCCAGAGCCAACTGCTGTGCCAGGGC90.10509107893507706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)