FastQCFastQC Report
Tue 10 May 2022
EGAF00003670240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8564
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1071.2494161606725829No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT640.7473143390938813No Hit
ACCATGTATCGTGCTCTTGCTAAAAGGCAGGCACAGTGATAGGCATTTGA320.37365716954694067No Hit
GGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAAAGCATCC310.3619803829985988No Hit
GATTAGATGTGGGCGCTACCAAATGTGAAGACAAGACAAGACTCTCAGGT280.3269500233535731No Hit
GTCTTGCTCTGTTGCCTAGGCTGGAGTGCAGTGGTGCGGTCTTGGCTCAC270.31527323680523117No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG240.2802428771602055No Hit
TTCCTGTAGTCTGCTGGTAAAGGATCCAAACAGGAGGGGACACTTTTGAC200.2335357309668379No Hit
GTGATAGGCATTTGAAATATCTCATGCTGTCTATCTAACTCACAACAATT200.2335357309668379No Hit
AGTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGA200.2335357309668379No Hit
GCTACTAAGCGGTAGAGTTGGGATTTGAACCTGCATTGTTCCAAAACGTT200.2335357309668379No Hit
CCTTAGAGGCTTATCATCCCCACAGGAGGACACCTGCCTAGTGGAGCTTT200.2335357309668379No Hit
TTATACGACTGCTTAATAATTCATATGCGCTACCCGGGAATCAATTCATA190.22185894441849605No Hit
ATTTCATACAGCTACTAAGCGGTAGAGTTGGGATTTGAACCTGCATTGTT190.22185894441849605No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT190.22185894441849605No Hit
ACGTATACTATCGACTCTAATTCCATTATCAGTCAAAAGTGTCCCCTCCT180.21018215787015412No Hit
GTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGAC170.19850537132181223No Hit
GGCTAAGATAATGTAGTTACCATGTATCGTGCTCTTGCTAAAAGGCAGGC170.19850537132181223No Hit
ATCTAACTCACAACAATTCCATAGGTAAGTACTTCCTTCCATCTCATTTT160.18682858477347034No Hit
GTCGTATAATATTTGACTTTCTCTCTTGTTTTACAGGCAATCTGTGAGAA150.17515179822512844No Hit
CTTTTATCATTCCTAACGCCCTCATGGTGGTGGTGATTTCTTTCCATTCC150.17515179822512844No Hit
CCCTCATGGTGGTGGTGATTTCTTTCCATTCCTGTAGTCTGCTGGTAAAG150.17515179822512844No Hit
CTATTTTTTCTACTTCCAAAACAAACCCTGAGAACAAGGCAGCCAAGAAG150.17515179822512844No Hit
TCCTAGAGGAGTCAAATTCATAGAAAGGAGAATCGTGGTGGGCATGGCTG140.16347501167678655No Hit
GGCTGGAGTGCAGTGGCGTGATCTTGGCTCACCGCAGCCTCCACCTCCTG140.16347501167678655No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA140.16347501167678655No Hit
CATATAGACAAATCCCTGGAAATTCAACAGAGAACAATCCAAAAGATTTG140.16347501167678655No Hit
GCTGGTAAGTATGCCATGATCATTCAGATGCCTCATTTCCTCCTGGGCGT140.16347501167678655No Hit
ACCAAATGTGAAGACAAGACAAGACTCTCAGGTTTCTGGGTAGGAAGTCC140.16347501167678655No Hit
CCTGTAGTCTGCTGGTAAAGGATCCAAACAGGAGGGGACACTTTTGACTG140.16347501167678655No Hit
GTCTAAAGGTGGGCATTAGCAGTCGGCATCTCACTGCTATCACTGTGGAT130.15179822512844465No Hit
GCACAAGCAAATGCCCTATTTCCTCTAACTATCCTTAGGGTCAAAGTCCA130.15179822512844465No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT130.15179822512844465No Hit
ATAGTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAA120.14012143858010276No Hit
TTCCATAGGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAA120.14012143858010276No Hit
ACCTGTGACTATCCAAGGCTGGGCACGGTGGCTCATGCCTGTAATACCAG120.14012143858010276No Hit
GCGGTAGAGTTGGGATTTGAACCTGCATTGTTCCAAAACGTTTCCTCTCT120.14012143858010276No Hit
TAGTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAG110.12844465203176086No Hit
ATCTCATGCTGTCTATCTAACTCACAACAATTCCATAGGTAAGTACTTCC110.12844465203176086No Hit
GTACAGGTCTAACTCATGATAGGTGCCCACTCAATGCTGGTTCTGCCACT110.12844465203176086No Hit
GAGATGGGGTTTCACCACGTTGGCCAGGTTGGTCTCGAACTCCTGACCTC110.12844465203176086No Hit
TTCTAAGGGGAAAAATAGCATCTTATATTAGGATAAAGGTGAAAGCATTT110.12844465203176086No Hit
ATGTTGCCCAGGCTGGTCTTGAACTTCTAACCTCAGGTGATCCACCAGTC110.12844465203176086No Hit
GAGTCTTGCTCTGTTGCCTAGGCTGGAGTGCAGTGGTGCGGTCTTGGCTC100.11676786548341896No Hit
GCCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACCGCAGCCTCCACC100.11676786548341896No Hit
AGACAAGACTCTCAGGTTTCTGGGTAGGAAGTCCGGATTGTGGTGCCTTG100.11676786548341896No Hit
CCACCATGTTGCCCAGGCTGGTCTTGAACTTCTAACCTCAGGTGATCCAC100.11676786548341896No Hit
GTCTATATGAATTGATTCCCGGGTAGCGCATATGAATTATTAAGCAGTCG100.11676786548341896No Hit
GATAATGTAGTTACCATGTATCGTGCTCTTGCTAAAAGGCAGGCACAGTG100.11676786548341896No Hit
ATATTATACGACTGCTTAATAATTCATATGCGCTACCCGGGAATCAATTC100.11676786548341896No Hit
CATCTAATCAGTCAACGTATACTATCGACTCTAATTCCATTATCAGTCAA100.11676786548341896No Hit
GTAGTGCAGTGGCGTGACCTCAGCTCACTGCAACCTCCGCCTCCTGGGAT100.11676786548341896No Hit
GGGTTCCACCATGTTGCCCAGGCTGGTCTTGAACTTCTAACCTCAGGTGA100.11676786548341896No Hit
GTCTATCTAACTCACAACAATTCCATAGGTAAGTACTTCCTTCCATCTCA100.11676786548341896No Hit
GTACTGACTTGAGGGAACCCCCTAAGGTCAAAGAACCTAGGATGTAAGCC90.10509107893507706No Hit
GACTATAGGCGTGAGCCACCATGCCTGACCATAAATGCTCTTTTATCATT90.10509107893507706No Hit
CTACAGGGACGTGCCACCACACTGACTAATTTTTTATTTTTAGTAGAGAT90.10509107893507706No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG90.10509107893507706No Hit
TCTTATATTAGGATAAAGGTGAAAGCATTTTCATAGTTGAAGCAAATCTT90.10509107893507706No Hit
GTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC90.10509107893507706No Hit
CTCCAACTACAGCTGCAGTTCCTGTTATGACCTGCTTCTATCCCAATCAG90.10509107893507706No Hit
GAATTAGAGTCGATAGTATACGTTGACTGATTAGATGTGGGCGCTACCAA90.10509107893507706No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG90.10509107893507706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.10509107893507706No Hit
TCTTAAGACAGGTTCTATGAGCACATGGTTTTGAGGGCTCAGCAAATCCC90.10509107893507706No Hit
GCCTAGGCTGGAGTGCAGTGGTGCGGTCTTGGCTCACTGCAAGCTCCGCC90.10509107893507706No Hit
CATGATAGGTGCCCACTCAATGCTGGTTCTGCCACTCAGCACAAGCAAAT90.10509107893507706No Hit
TACATAAGCTCACCTCACAGATTAGCCTGGGAAAGGAAAAAAAATTAAGA90.10509107893507706No Hit
GCTGGTAAAGGATCCAAACAGGAGGGGACACTTTTGACTGATAATGGAAT90.10509107893507706No Hit
CCACCACACTGACTAATTTTTTATTTTTAGTAGAGATGGGGTTTCACCAC90.10509107893507706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)