FastQCFastQC Report
Tue 10 May 2022
EGAF00003670242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8838
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1151.3011993663724826No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT951.0749038243946594No Hit
GGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAAAGCATCC400.4525910839556461No Hit
GTGATAGGCATTTGAAATATCTCATGCTGTCTATCTAACTCACAACAATT310.3507580900656257No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG290.3281285358678434No Hit
GTACAGGTCTAACTCATGATAGGTGCCCACTCAATGCTGGTTCTGCCACT290.3281285358678434No Hit
ACCATGTATCGTGCTCTTGCTAAAAGGCAGGCACAGTGATAGGCATTTGA290.3281285358678434No Hit
GATTAGATGTGGGCGCTACCAAATGTGAAGACAAGACAAGACTCTCAGGT260.29418420457116995No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT250.2828694274722788No Hit
TTATACGACTGCTTAATAATTCATATGCGCTACCCGGGAATCAATTCATA210.23761031907671418No Hit
GTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGAC180.20366598778004072No Hit
TTCCATAGGTAAGTACTTCCTTCCATCTCATTTTTCAGATAAGGAAACAA160.18103643358225843No Hit
GGCTAAGATAATGTAGTTACCATGTATCGTGCTCTTGCTAAAAGGCAGGC160.18103643358225843No Hit
TTATTAAGCAGTCGTATAATATTTGACTTTCTCTCTTGTTTTACAGGCAA150.1697216564833673No Hit
TTCCTGTAGTCTGCTGGTAAAGGATCCAAACAGGAGGGGACACTTTTGAC150.1697216564833673No Hit
AGTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGA150.1697216564833673No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT150.1697216564833673No Hit
TACATAAGCTCACCTCACAGATTAGCCTGGGAAAGGAAAAAAAATTAAGA150.1697216564833673No Hit
ATAGTATACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAA140.15840687938447612No Hit
ATCTCATGCTGTCTATCTAACTCACAACAATTCCATAGGTAAGTACTTCC140.15840687938447612No Hit
GACTATAGGCGTGAGCCACCATGCCTGACCATAAATGCTCTTTTATCATT140.15840687938447612No Hit
CCTTAGAGGCTTATCATCCCCACAGGAGGACACCTGCCTAGTGGAGCTTT140.15840687938447612No Hit
GGATAAAGGTGAAAGCATTTTCATAGTTGAAGCAAATCTTAAGACAGGTT130.14709210228558497No Hit
GCTCTGTTGCCTAGGCTGGAGTGCAGTGGTGCGGTCTTGGCTCACTGCAA130.14709210228558497No Hit
GAAACAAAGCATCCAAGATGTGAAATAATTTGCCAATTTCATACAGCTAC130.14709210228558497No Hit
TCCTAGAGGAGTCAAATTCATAGAAAGGAGAATCGTGGTGGGCATGGCTG120.1357773251866938No Hit
GCTGGTAAAGGATCCAAACAGGAGGGGACACTTTTGACTGATAATGGAAT120.1357773251866938No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA110.12446254808780267No Hit
ACAGAGAACAATCCAAAAGATTTGCTCCTTGGTCCATGGTATGAAAAATG110.12446254808780267No Hit
ATTTCATACAGCTACTAAGCGGTAGAGTTGGGATTTGAACCTGCATTGTT110.12446254808780267No Hit
GCCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACCGCAGCCTCCACC110.12446254808780267No Hit
TCTGTGAGGTGAGCTTATGTAAGATCAACAGCAAAGTTCTAAAGAGTGGT110.12446254808780267No Hit
GGCTGGAGTGCAGTGGCGTGATCTTGGCTCACCGCAGCCTCCACCTCCTG110.12446254808780267No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG110.12446254808780267No Hit
TCTTAAGACAGGTTCTATGAGCACATGGTTTTGAGGGCTCAGCAAATCCC110.12446254808780267No Hit
ACCAAATGTGAAGACAAGACAAGACTCTCAGGTTTCTGGGTAGGAAGTCC110.12446254808780267No Hit
CCTCTAAGGTGTATGTAAGAAAGTTTGGAAGTTTTAGGTCAAAATGTGGT110.12446254808780267No Hit
GTAGAGAGGGGGTTCCACCATGTTGCCCAGGCTGGTCTTGAACTTCTAAC110.12446254808780267No Hit
CTAACTATCCTTAGGGTCAAAGTCCATGGTGACTAGTGAATCACATCTAA110.12446254808780267No Hit
GTCTCGTTCTGTCTCCCAGGCTGTAGTGCAGTGGCGTGACCTCAGCTCAC100.11314777098891153No Hit
CTATCTAACTCACAACAATTCCATAGGTAAGTACTTCCTTCCATCTCATT100.11314777098891153No Hit
AGACAAGACTCTCAGGTTTCTGGGTAGGAAGTCCGGATTGTGGTGCCTTG100.11314777098891153No Hit
CTACAGGGACGTGCCACCACACTGACTAATTTTTTATTTTTAGTAGAGAT100.11314777098891153No Hit
CATATAGACAAATCCCTGGAAATTCAACAGAGAACAATCCAAAAGATTTG100.11314777098891153No Hit
TCTCTCTTGTTGCCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACCG100.11314777098891153No Hit
GAGATGGGGTTTCACCACGTTGGCCAGGTTGGTCTCGAACTCCTGACCTC100.11314777098891153No Hit
GCTAAAAGGCAGGCACAGTGATAGGCATTTGAAATATCTCATGCTGTCTA100.11314777098891153No Hit
TTTTAGTAGAGAGGGGGTTCCACCATGTTGCCCAGGCTGGTCTTGAACTT100.11314777098891153No Hit
GCTACTAAGCGGTAGAGTTGGGATTTGAACCTGCATTGTTCCAAAACGTT100.11314777098891153No Hit
AACTATCCTTAGGGTCAAAGTCCATGGTGACTAGTGAATCACATCTAAAT100.11314777098891153No Hit
CCACCACACTGACTAATTTTTTATTTTTAGTAGAGATGGGGTTTCACCAC100.11314777098891153No Hit
CCATAGTACAGGTCTAACTCATGATAGGTGCCCACTCAATGCTGGTTCTG100.11314777098891153No Hit
TACGTTGACTGATTAGATGTGGGCGCTACCAAATGTGAAGACAAGACAAG90.10183299389002036No Hit
TCCAAGATGTGAAATAATTTGCCAATTTCATACAGCTACTAAGCGGTAGA90.10183299389002036No Hit
GTCTTGCTCTGTTGCCTAGGCTGGAGTGCAGTGGTGCGGTCTTGGCTCAC90.10183299389002036No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT90.10183299389002036No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC90.10183299389002036No Hit
GTATCGTGCTCTTGCTAAAAGGCAGGCACAGTGATAGGCATTTGAAATAT90.10183299389002036No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG90.10183299389002036No Hit
GGTCTAACTCATGATAGGTGCCCACTCAATGCTGGTTCTGCCACTCAGCA90.10183299389002036No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT90.10183299389002036No Hit
CTTTTATCATTCCTAACGCCCTCATGGTGGTGGTGATTTCTTTCCATTCC90.10183299389002036No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT90.10183299389002036No Hit
GTACATGGGCCAGCAGAAGGTCGCGCACCGCGCCGCCCACCACGTAGGCC90.10183299389002036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.10183299389002036No Hit
ACGTATACTATCGACTCTAATTCCATTATCAGTCAAAAGTGTCCCCTCCT90.10183299389002036No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT90.10183299389002036No Hit
GACTACAGGCGGCCGCCACCGCGCCTGGCTAATTTTTTGTATTTTTAGTA90.10183299389002036No Hit
CATTTATACTCCTACCAGCAATATGTGAAAGTATTTATTGGCATGATTTA90.10183299389002036No Hit
CTACAGGAATGGAAAGAAATCACCACCACCATGAGGGCGTTAGGAATGAT90.10183299389002036No Hit
GCGGTAGAGTTGGGATTTGAACCTGCATTGTTCCAAAACGTTTCCTCTCT90.10183299389002036No Hit
ATCTCACACCAGTCAGAATAGCAATTATTAATATTAAAAAGTCCAAAAAC90.10183299389002036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)