FastQCFastQC Report
Tue 10 May 2022
EGAF00003670325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19752
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAGCGTGGTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCA640.3240178209801539No Hit
GTACATGGGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCC620.3138922640745241No Hit
CCCTCAGAGAGTCCACTTCACTCCCCTGCTACCTCCACCAGAGCAGGTGC550.27845281490481977No Hit
GCTTAAAACATGTCTCTGCATTTTATTTTAAGAAAACACAAACCTGGTCA530.26832725799918994No Hit
GGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCCCTGGCAA480.24301336573511542No Hit
GCCTTTGTGTTGGTTGGCCTCCAGCCAGGAGGTGGCGCTTTCAAGAGTAC450.2278250303766707No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA430.2176994734710409Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG410.2075739165654111No Hit
GAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAGGTTCT390.19744835965978128No Hit
CTCTGAGGGTTATTGGTTATGTATTTGTTTGTTTAGTACACTGCCTTTGT370.18732280275415147No Hit
GGGTAGAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAG370.18732280275415147No Hit
GTCCTATAGGGAAGATGCAGACTTGCCCTAGGGCAAGTATTCAGGTTTCC360.18226002430133656No Hit
GTACATGGGAGGCTAAATGCAAAGTCATCTCTTGAGTCATGAGCTGGACT340.17213446739570676No Hit
GTACATGGGAGTTCCTGTGCTGGAGAGAAAGGCAGTCAGAGGAGAAAATG340.17213446739570676No Hit
GTACAAGCCTGGATCCCAGTAGCTCAGCTGGGTGGCTAGACCCAGAGGAG340.17213446739570676No Hit
GTGTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGTTTTTCTGGTTC330.16707168894289187No Hit
GTTTAGTACACTGCCTTTGTGTTGGTTGGCCTCCAGCCAGGAGGTGGCGC330.16707168894289187No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG320.16200891049007696No Hit
GGTCTACACTGCCAACCAGAACTGTGTAGATGCGTACCCCACTTTCCTCG310.15694613203726204No Hit
GTTTTATCGTATTACCAGAATTGTTTTTCTGGTTCTTTCTCATTTGGGTA300.15188335358444716No Hit
ACACAAACCTGGTCACAAAACATCTTCAGAGAACAGGATAAGTGAAGAAA300.15188335358444716No Hit
TCTTTATGTAGTTTTCAAAGTCACTTCCGAAAAAGAAGATGAGGTAATAG280.14175779667881733No Hit
GTACTAAACAAACAAATACATAACCAATAACCCTCAGAGAGTCCACTTCA280.14175779667881733No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT280.14175779667881733No Hit
TCTGAGGATCGGTCAGAGCCCAAGACAGGAAGAAGTTTGAGAAGCCCCAG270.13669501822600244No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA260.13163223977318753No Hit
CTATAGGACAGCACCTGATGTACTCTTGAAAGCGCCACCTCCTGGCTGGA250.1265694613203726No Hit
GAGTACATGGGAGCGTGGTCCAGAATGGATTCTTTGCCCATAAAGTGGAG240.12150668286755771No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT240.12150668286755771Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCACGAAAGCAGGACCCA240.12150668286755771No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCC240.12150668286755771No Hit
GCTTAATAATCAACCTTCTGAATTCTTTTTCTGGCAATTCAGAGATTTTT240.12150668286755771No Hit
GTATCAACGCAGAGTACATGGGAGTGGGGTGAGGCCTGTGACTGCTGGCT230.11644390441474281No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG230.11644390441474281Clontech Universal Primer Mix Long (96% over 26bp)
CACAAGGAGAGACTGAGCTCAGACACCCTATTTCTGCCCTCACCTGGTGG230.11644390441474281No Hit
GGCTTGTACTGTGGAGTGTCTGCAAAGTGTCCTGTGATGTGATCCATCTT220.11138112596192791No Hit
ATTTATGACTCAGCAGAGGCAGCCATAATCCCCCGGGAACATAATTCCAC220.11138112596192791No Hit
ATAAAGTGGAGCACGAAAGCAGGACCCAGAATGGGAGGAGCTTCCAGAGG210.106318347509113No Hit
ATATTTTGGTGGTGTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGT210.106318347509113No Hit
ATGCTAACAGGGACGCAGACACATTCAGGTGCCTTTGCAGAAAGAGATGC210.106318347509113No Hit
GTACACTGCCTTTGTGTTGGTTGGCCTCCAGCCAGGAGGTGGCGCTTTCA200.1012555690562981No Hit
ATAATCAACCTTCTGAATTCTTTTTCTGGCAATTCAGAGATTTTTTCTGC200.1012555690562981No Hit
GTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGTTTTTCTGGTTCTT200.1012555690562981No Hit
TTCAAGAGTACATCAGGTGCTGTCCTATAGGGAAGATGCAGACTTGCCCT200.1012555690562981No Hit
GTATAGAATTTGTCACAGTGGAACGTAATTGTCACTGGGAGGTGAGTTTA200.1012555690562981No Hit
TATCAACGCAGAGTACATGGGAGTGGGGTGAGGCCTGTGACTGCTGGCTT200.1012555690562981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)