FastQCFastQC Report
Tue 10 May 2022
EGAF00003670326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19752
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAGCGTGGTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCA730.36958282705548806No Hit
GTACATGGGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCC560.28351559335763465No Hit
CCCTCAGAGAGTCCACTTCACTCCCCTGCTACCTCCACCAGAGCAGGTGC530.26832725799918994No Hit
GCTTAAAACATGTCTCTGCATTTTATTTTAAGAAAACACAAACCTGGTCA520.26326447954637505No Hit
GGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCCCTGGCAA440.22276225192385582No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT430.2176994734710409Clontech Universal Primer Mix Long (96% over 26bp)
GTACAAGCCTGGATCCCAGTAGCTCAGCTGGGTGGCTAGACCCAGAGGAG430.2176994734710409No Hit
GTACTAAACAAACAAATACATAACCAATAACCCTCAGAGAGTCCACTTCA400.2025111381125962No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT360.18226002430133656No Hit
GTACATGGGAGTTCCTGTGCTGGAGAGAAAGGCAGTCAGAGGAGAAAATG350.17719724584852167No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA340.17213446739570676Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG330.16707168894289187No Hit
GTTTAGTACACTGCCTTTGTGTTGGTTGGCCTCCAGCCAGGAGGTGGCGC330.16707168894289187No Hit
GTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCACGAAAGCAGGACCCA300.15188335358444716No Hit
CTATAGGACAGCACCTGATGTACTCTTGAAAGCGCCACCTCCTGGCTGGA290.14682057513163224No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG290.14682057513163224No Hit
GAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAGGTTCT280.14175779667881733No Hit
GTCCTATAGGGAAGATGCAGACTTGCCCTAGGGCAAGTATTCAGGTTTCC280.14175779667881733No Hit
GTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGTTTTTCTGGTTCTT260.13163223977318753No Hit
GGTCTACACTGCCAACCAGAACTGTGTAGATGCGTACCCCACTTTCCTCG250.1265694613203726No Hit
GCCTTTGTGTTGGTTGGCCTCCAGCCAGGAGGTGGCGCTTTCAAGAGTAC240.12150668286755771No Hit
GTACATGGGAGGCTAAATGCAAAGTCATCTCTTGAGTCATGAGCTGGACT240.12150668286755771No Hit
CTCCACAGTACAAGCCTGGATCCCAGTAGCTCAGCTGGGTGGCTAGACCC240.12150668286755771No Hit
CTCTGAGGGTTATTGGTTATGTATTTGTTTGTTTAGTACACTGCCTTTGT230.11644390441474281No Hit
AGGAGAGACTGAGCTCAGACACCCTATTTCTGCCCTCACCTGGTGGTCTT220.11138112596192791No Hit
GTTTTATCGTATTACCAGAATTGTTTTTCTGGTTCTTTCTCATTTGGGTA220.11138112596192791No Hit
GGGTAGAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAG220.11138112596192791No Hit
CCACAGTACAAGCCTGGATCCCAGTAGCTCAGCTGGGTGGCTAGACCCAG210.106318347509113No Hit
ATCTGGGAGGGTGGACTTGCCTCACATGGCTTTGAGTCAAGATGGTAGAA210.106318347509113No Hit
TCAGAGAACAGGATAAGTGAAGAAACAAACAAAATGCATGGGAATAGCAA210.106318347509113No Hit
CCCCAAATCTGATACACAACTTCCTTTCATCTACAGGACAACTGGGCAAG200.1012555690562981No Hit
ATTTATGACTCAGCAGAGGCAGCCATAATCCCCCGGGAACATAATTCCAC200.1012555690562981No Hit
GTACATGGGAGAAGTGGGCAGCTGTGGTGGTGCCTTCTGGAGAAGAGCAG200.1012555690562981No Hit
GCTTAATAATCAACCTTCTGAATTCTTTTTCTGGCAATTCAGAGATTTTT200.1012555690562981No Hit
ACGTAATTGTCACTGGGAGGTGAGTTTATTCAGGGCAGGGATCTTGTTTT200.1012555690562981No Hit
GGTATGATATTTTGGTGGTGTTAAAGGACCTTGTTTTATCGTATTACCAG200.1012555690562981No Hit
ATCTTACTCTGCTCCAAGGCCACAGACCACATCCCCAAATCTGATACACA200.1012555690562981No Hit
GTGTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGTTTTTCTGGTTC200.1012555690562981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)