FastQCFastQC Report
Tue 10 May 2022
EGAF00003670327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20200
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCC810.400990099009901No Hit
CCCTCAGAGAGTCCACTTCACTCCCCTGCTACCTCCACCAGAGCAGGTGC660.32673267326732675No Hit
GTACATGGGAGCGTGGTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCA660.32673267326732675No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA500.24752475247524752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT440.21782178217821785Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGTTCCTGTGCTGGAGAGAAAGGCAGTCAGAGGAGAAAATG440.21782178217821785No Hit
GTTTAGTACACTGCCTTTGTGTTGGTTGGCCTCCAGCCAGGAGGTGGCGC420.20792079207920794No Hit
GTACAAGCCTGGATCCCAGTAGCTCAGCTGGGTGGCTAGACCCAGAGGAG410.20297029702970298No Hit
GTACATGGGAGGCTAAATGCAAAGTCATCTCTTGAGTCATGAGCTGGACT380.18811881188118812No Hit
GTGTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGTTTTTCTGGTTC380.18811881188118812No Hit
GCTTAAAACATGTCTCTGCATTTTATTTTAAGAAAACACAAACCTGGTCA360.17821782178217824No Hit
GGGTAGAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAG330.16336633663366337No Hit
ATCTTACTCTGCTCCAAGGCCACAGACCACATCCCCAAATCTGATACACA330.16336633663366337No Hit
GGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCCCTGGCAA320.15841584158415842No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT310.15346534653465346No Hit
GTTTTATCGTATTACCAGAATTGTTTTTCTGGTTCTTTCTCATTTGGGTA280.1386138613861386No Hit
CTATAGGACAGCACCTGATGTACTCTTGAAAGCGCCACCTCCTGGCTGGA280.1386138613861386No Hit
GAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAGGTTCT280.1386138613861386No Hit
ACACAAACCTGGTCACAAAACATCTTCAGAGAACAGGATAAGTGAAGAAA280.1386138613861386No Hit
GTACTAAACAAACAAATACATAACCAATAACCCTCAGAGAGTCCACTTCA280.1386138613861386No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG270.13366336633663367No Hit
GTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGTTTTTCTGGTTCTT270.13366336633663367No Hit
GGTATGATATTTTGGTGGTGTTAAAGGACCTTGTTTTATCGTATTACCAG270.13366336633663367No Hit
CTCTGAGGGTTATTGGTTATGTATTTGTTTGTTTAGTACACTGCCTTTGT260.12871287128712872No Hit
CTCTACGGCCCTGCCCACTCCTGGGAAACCTGAATACTTGCCCTAGGGCA250.12376237623762376No Hit
GTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCACGAAAGCAGGACCCA250.12376237623762376No Hit
GGTCTACACTGCCAACCAGAACTGTGTAGATGCGTACCCCACTTTCCTCG240.1188118811881188No Hit
CATTTATGACTCAGCAGAGGCAGCCATAATCCCCCGGGAACATAATTCCA240.1188118811881188No Hit
TTCCAATTCCAATCGGGCTTCTGAGAAACCCAAAGTACCCAACCCCTGGG240.1188118811881188No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG240.1188118811881188No Hit
CCCCCAGTCTGTGGCATAGGTCTTGGTACATGTGGTTCATGACATTGCAC230.11386138613861385No Hit
TATCAACGCAGAGTACATGGGAGCGTGGTCCAGAATGGATTCTTTGCCCA230.11386138613861385No Hit
GAGTTTATTCAGGGCAGGGATCTTGTTTTATTCTCCTTGTACCCCCAGTC230.11386138613861385No Hit
GTATCAACGCAGAGTACATGGGAGGCTAAATGCAAAGTCATCTCTTGAGT230.11386138613861385No Hit
GTCCTATAGGGAAGATGCAGACTTGCCCTAGGGCAAGTATTCAGGTTTCC230.11386138613861385No Hit
ATAATCAACCTTCTGAATTCTTTTTCTGGCAATTCAGAGATTTTTTCTGC220.10891089108910892No Hit
GTATTTGTTTGTTTAGTACACTGCCTTTGTGTTGGTTGGCCTCCAGCCAG220.10891089108910892No Hit
ATATTTTGGTGGTGTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGT220.10891089108910892No Hit
GGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCCCTGGCAACATTTAT220.10891089108910892No Hit
TCTTTATGTAGTTTTCAAAGTCACTTCCGAAAAAGAAGATGAGGTAATAG210.10396039603960397No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT210.10396039603960397No Hit
GAATTTGTCACAGTGGAACGTAATTGTCACTGGGAGGTGAGTTTATTCAG210.10396039603960397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)