FastQCFastQC Report
Tue 10 May 2022
EGAF00003670328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20200
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAGCGTGGTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCA770.3811881188118812No Hit
CCCTCAGAGAGTCCACTTCACTCCCCTGCTACCTCCACCAGAGCAGGTGC550.27227722772277224No Hit
GTACATGGGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCC550.27227722772277224No Hit
GCTTAAAACATGTCTCTGCATTTTATTTTAAGAAAACACAAACCTGGTCA440.21782178217821785No Hit
CTCTGAGGGTTATTGGTTATGTATTTGTTTGTTTAGTACACTGCCTTTGT410.20297029702970298No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT380.18811881188118812Clontech Universal Primer Mix Long (96% over 26bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG360.17821782178217824No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA360.17821782178217824Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAGTGGGGTGAGGCCTGTGACTGCTGGCTTTCCTCTACTTCCCTGGCAA360.17821782178217824No Hit
GTACATGGGAGGCTAAATGCAAAGTCATCTCTTGAGTCATGAGCTGGACT360.17821782178217824No Hit
GTGTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGTTTTTCTGGTTC360.17821782178217824No Hit
GGGTAGAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAG350.17326732673267328No Hit
CTATAGGACAGCACCTGATGTACTCTTGAAAGCGCCACCTCCTGGCTGGA340.16831683168316833No Hit
GTTTAGTACACTGCCTTTGTGTTGGTTGGCCTCCAGCCAGGAGGTGGCGC340.16831683168316833No Hit
GTACAAGCCTGGATCCCAGTAGCTCAGCTGGGTGGCTAGACCCAGAGGAG330.16336633663366337No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG320.15841584158415842No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT320.15841584158415842No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA310.15346534653465346No Hit
GAGTATGTCAGAGGAAAGATCTGGGACTCAAGGGCTGCTGCTCAGGTTCT310.15346534653465346No Hit
GTATCAACGCAGAGTACATGGGAGCGTGGTCCAGAATGGATTCTTTGCCC300.1485148514851485No Hit
ATCTTACTCTGCTCCAAGGCCACAGACCACATCCCCAAATCTGATACACA300.1485148514851485No Hit
GTACTAAACAAACAAATACATAACCAATAACCCTCAGAGAGTCCACTTCA270.13366336633663367No Hit
GTACATGGGAGTTCCTGTGCTGGAGAGAAAGGCAGTCAGAGGAGAAAATG250.12376237623762376No Hit
GTCCAGAATGGATTCTTTGCCCATAAAGTGGAGCACGAAAGCAGGACCCA250.12376237623762376No Hit
ATATTTTGGTGGTGTTAAAGGACCTTGTTTTATCGTATTACCAGAATTGT240.1188118811881188No Hit
GTTTTATCGTATTACCAGAATTGTTTTTCTGGTTCTTTCTCATTTGGGTA230.11386138613861385No Hit
ATTTATGACTCAGCAGAGGCAGCCATAATCCCCCGGGAACATAATTCCAC230.11386138613861385No Hit
TTCCAATTCCAATCGGGCTTCTGAGAAACCCAAAGTACCCAACCCCTGGG220.10891089108910892No Hit
GGACTGGGAACGACATCGCCATCCCCCCACAGCAGCCACAGCAAGCCCCA220.10891089108910892No Hit
GTACATGGGAGAAGTGGGCAGCTGTGGTGGTGCCTTCTGGAGAAGAGCAG220.10891089108910892No Hit
GGTATGATATTTTGGTGGTGTTAAAGGACCTTGTTTTATCGTATTACCAG210.10396039603960397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)