FastQCFastQC Report
Tue 10 May 2022
EGAF00003670350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302590
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6650.21976932482897651No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT5580.1844079447437126No Hit
GGTCTATAGAGCTCACACCCATACTCCCAGCTGGGACCCAGCCTTCCCTG5150.1701972966720645No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC4680.15466472784956542No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG4630.1530123269110017No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4360.14408936184275753No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA4220.13946263921477908No Hit
ACTCAGGACACTCCCGAGTTTTTTATAGACCAAGGCCATGCCAAGGTGGC3710.122608149641429No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG3670.12128622889057801No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC3570.11798142701345055No Hit
CTCTCAGGAAGCACAGAGTCCAACAGGACCATGAATTCTTCTGGAGAGAG3500.11566806569946132No Hit
TCCTGAGAGTGCCCATCGGCTGAAGTCAAGCATCGGGCTGATCAATGAAA3390.1120327836346211No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA3210.10608414025579166No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG3110.1027793383786642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)