FastQCFastQC Report
Tue 10 May 2022
EGAF00003670352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338304
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6770.2001158721150208No Hit
GGTCTATAGAGCTCACACCCATACTCCCAGCTGGGACCCAGCCTTCCCTG6650.19656876655315927No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT6430.1900657396897465No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5310.1569594211123723No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG5220.15429909194097616No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA4850.1433621831252365No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4820.1424754067347711No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG4250.12562665531592887No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC4050.11971481271282633No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC3760.11114264093832767No Hit
ACTCAGGACACTCCCGAGTTTTTTATAGACCAAGGCCATGCCAAGGTGGC3750.11084704880817253No Hit
TCCTGAGAGTGCCCATCGGCTGAAGTCAAGCATCGGGCTGATCAATGAAA3670.10848231176693152No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG3600.10641316685584562No Hit
CTCTCAGGAAGCACAGAGTCCAACAGGACCATGAATTCTTCTGGAGAGAG3500.10345724555429435No Hit
TCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTG3500.10345724555429435No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA3410.10079691638289821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)