FastQCFastQC Report
Tue 10 May 2022
EGAF00003670427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24195
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG740.30584831576772065No Hit
GTACATGGGAGACAATGGACCTGAGACCATGCGTATGTCCCGAGGCGGCT670.2769167183302335No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT580.23971895019632156No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA510.21078735275883448No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC480.19838809671419716No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA390.1611903285802852No Hit
CAATGGACCTGAGACCATGCGTATGTCCCGAGGCGGCTGCTCCGGTCTCT390.1611903285802852No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG390.1611903285802852No Hit
GATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACG390.1611903285802852No Hit
GTACATGGGATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACT370.15292415788386032No Hit
GTATCAACGCAGAGTACATGGGATCCAGACCGTCGGCTGATCGTGGTTTT360.14879107253564788No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG350.14465798718743542No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA340.14052490183922298No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT340.14052490183922298No Hit
GTACATGGGAGGATGCTGCAGCGACTCCTACAAAGGACGTAAAAGGATGG330.13639181649101054No Hit
GTATCAACGCAGAGTACATGGGAGACAATGGACCTGAGACCATGCGTATG320.1322587311427981No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT310.12812564579458566No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA310.12812564579458566No Hit
GGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCC310.12812564579458566No Hit
ATATAGGTCAGGCCTTGCAGTGGCACAGTATGTGACCCTAGTTTCTTTAC310.12812564579458566No Hit
ACCTGAGACCATGCGTATGTCCCGAGGCGGCTGCTCCGGTCTCTTGCGGT300.12399256044637322No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC300.12399256044637322No Hit
GAGTACATGGGATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA290.11985947509816078No Hit
GTACATGGGAAGGTGCTTCAACCCACTGTCTTCCCCGTGGTTCCAAGACT290.11985947509816078No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG290.11985947509816078No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA280.11572638974994834No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT280.11572638974994834No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT280.11572638974994834No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA270.1115933044017359No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC270.1115933044017359No Hit
GTATGTGACCCTAGTTTCTTTACTGTTACTTTCCTTTTGGTCATGCTACA270.1115933044017359No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC270.1115933044017359No Hit
ACCTACGAGGGCGGTGTCCGAGAGCCTGCCTTGGCCTTCTGGCCAGGTCA250.10332713370531102No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG250.10332713370531102No Hit
ACATTATGATGACTACAATGTCAGTGGACAATGGAATTTTTCAGCTCCAT250.10332713370531102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)