FastQCFastQC Report
Tue 10 May 2022
EGAF00003670428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24195
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC800.3306468278569953No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT580.23971895019632156No Hit
GTACATGGGAGACAATGGACCTGAGACCATGCGTATGTCCCGAGGCGGCT500.20665426741062204No Hit
ACCTGAGACCATGCGTATGTCCCGAGGCGGCTGCTCCGGTCTCTTGCGGT500.20665426741062204No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT480.19838809671419716No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG470.19425501136598472No Hit
GTACATGGGATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACT470.19425501136598472No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA470.19425501136598472No Hit
CAATGGACCTGAGACCATGCGTATGTCCCGAGGCGGCTGCTCCGGTCTCT400.16532341392849764No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA380.15705724323207276No Hit
GTATGTGACCCTAGTTTCTTTACTGTTACTTTCCTTTTGGTCATGCTACA370.15292415788386032No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG370.15292415788386032No Hit
GTATCAACGCAGAGTACATGGGATCCAGACCGTCGGCTGATCGTGGTTTT360.14879107253564788No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA360.14879107253564788No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG350.14465798718743542No Hit
GTACATGGGAAGGTGCTTCAACCCACTGTCTTCCCCGTGGTTCCAAGACT350.14465798718743542No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT330.13639181649101054No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT310.12812564579458566No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC310.12812564579458566No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT310.12812564579458566No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA300.12399256044637322No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG300.12399256044637322No Hit
GTATCAACGCAGAGTACATGGGAGACAATGGACCTGAGACCATGCGTATG280.11572638974994834No Hit
GTGTGTAACCAGCCGTCTTTGTCCAAAGCTTCTGCTGTTTTCGCTGGGTC280.11572638974994834No Hit
GATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACG280.11572638974994834No Hit
ATTTTACATGGTGTACTTTTATAAGACCAGCAATACAGTAGGTTTGTTTA260.10746021905352346No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC260.10746021905352346No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA260.10746021905352346No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA260.10746021905352346No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT250.10332713370531102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)