FastQCFastQC Report
Tue 10 May 2022
EGAF00003670429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24684
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG770.31194295900178254No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC740.2997893372224923No Hit
GTACATGGGAGACAATGGACCTGAGACCATGCGTATGTCCCGAGGCGGCT650.2633284718846216No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT580.23497002106627773No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA500.20256036298817048No Hit
ACCTGAGACCATGCGTATGTCCCGAGGCGGCTGCTCCGGTCTCTTGCGGT490.19850915572840705No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG490.19850915572840705No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT480.19445794846864364No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA440.17825311942959002No Hit
GTACATGGGATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACT440.17825311942959002No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT430.1742019121698266No Hit
GATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACG410.1660994976502998No Hit
CAATGGACCTGAGACCATGCGTATGTCCCGAGGCGGCTGCTCCGGTCTCT390.15799708313077296No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG390.15799708313077296No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC380.15394587587100958No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA350.14179225409171933No Hit
GTTGTATATGTGGCCCATCGCTGACCAAAACATCATTATATGGCACATGA340.13774104683195593No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT330.1336898395721925No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA310.12558742505266568No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG310.12558742505266568No Hit
TATCAACGCAGAGTACATGGGATCCAGACCGTCGGCTGATCGTGGTTTTA300.12153621779290229No Hit
GGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAG300.12153621779290229No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC300.12153621779290229No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC290.11748501053313887No Hit
GTACATGGGAGGATGCTGCAGCGACTCCTACAAAGGACGTAAAAGGATGG290.11748501053313887No Hit
ATATAGGTCAGGCCTTGCAGTGGCACAGTATGTGACCCTAGTTTCTTTAC290.11748501053313887No Hit
GTATCAACGCAGAGTACATGGGAGACAATGGACCTGAGACCATGCGTATG280.11343380327337546No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA280.11343380327337546No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG280.11343380327337546No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA280.11343380327337546No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT280.11343380327337546No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA270.10938259601361207No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT270.10938259601361207No Hit
GTACATGGGAAGGTGCTTCAACCCACTGTCTTCCCCGTGGTTCCAAGACT270.10938259601361207No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT260.10533138875384865No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT260.10533138875384865No Hit
ACCTACGAGGGCGGTGTCCGAGAGCCTGCCTTGGCCTTCTGGCCAGGTCA260.10533138875384865No Hit
TTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCT250.10128018149408524No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA250.10128018149408524No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA250.10128018149408524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)