FastQCFastQC Report
Tue 10 May 2022
EGAF00003670430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24684
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC630.2552260573650948No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT620.25117485010533136No Hit
GTACATGGGAGACAATGGACCTGAGACCATGCGTATGTCCCGAGGCGGCT580.23497002106627773No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG540.21876519202722414No Hit
ACCTGAGACCATGCGTATGTCCCGAGGCGGCTGCTCCGGTCTCTTGCGGT540.21876519202722414No Hit
CAATGGACCTGAGACCATGCGTATGTCCCGAGGCGGCTGCTCCGGTCTCT430.1742019121698266No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT420.1701507049100632No Hit
GTACATGGGATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACT410.1660994976502998No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG400.1620482903905364No Hit
GTATGTGACCCTAGTTTCTTTACTGTTACTTTCCTTTTGGTCATGCTACA390.15799708313077296No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG390.15799708313077296No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT370.14989466861124615No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG370.14989466861124615No Hit
ATATAGGTCAGGCCTTGCAGTGGCACAGTATGTGACCCTAGTTTCTTTAC370.14989466861124615No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT350.14179225409171933No Hit
GTATCAACGCAGAGTACATGGGATCCAGACCGTCGGCTGATCGTGGTTTT340.13774104683195593No Hit
CCTCTGGGGTGGCCCCGGCCACACCCCCCAGCAGGTTGTAGTTCTCACCA340.13774104683195593No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA330.1336898395721925No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA330.1336898395721925No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT330.1336898395721925No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA320.12963863231242911No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC300.12153621779290229No Hit
GTATCAACGCAGAGTACATGGGAGACAATGGACCTGAGACCATGCGTATG300.12153621779290229No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC300.12153621779290229No Hit
TCCTTGGACAGGTCATAGAGCAGCGGGGGCTCATGAGCAGTCAGAGAGCT290.11748501053313887No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA290.11748501053313887No Hit
ATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGT290.11748501053313887No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT280.11343380327337546No Hit
ATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGAC270.10938259601361207No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT270.10938259601361207No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCC270.10938259601361207No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG270.10938259601361207No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA270.10938259601361207No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG270.10938259601361207No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGC260.10533138875384865No Hit
GATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACG260.10533138875384865No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG250.10128018149408524No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC250.10128018149408524No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA250.10128018149408524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)