FastQCFastQC Report
Tue 7 May 2024
EGAF00003670486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37692
Total Bases3.7 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG620.16449113870317308No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG610.16183805582086383No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA590.15653189005624535No Hit
GAATTAACCCGAGCAGGCATGGAGGCCTCTGCTCTCACCTCATCAGCAGT490.13000106123315294No Hit
GTACATGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAG480.1273479783508437No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA450.11938872970391595No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC440.11673564682160671No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT430.11408256393929746No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG430.11408256393929746No Hit
CTCTAGGCCACGGAATTAACCCGAGCAGGCATGGAGGCCTCTGCTCTCAC420.11142948105698822No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG410.10877639817467898No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT400.10612331529236974No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT390.10347023241006048No Hit
ATTTGGGATAGTTGGCTCCTCGCTGGGTCAGGATGGCTGGGTGCCTTCTC380.10081714952775125No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT380.10081714952775125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)