FastQCFastQC Report
Tue 10 May 2022
EGAF00003670501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390985
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAACACGGCACATAAAAAAGATTAAATTAAGAGAAGGGACAGGAACT11460.29310587362686547No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG8680.2220034016650255No Hit
TCCCCATAGGGGACACTTATCCTTTGGCTAAACTAATATAAATAATGGAA7990.2043556658183818No Hit
ACCTAATACAATAACACGGCACATAAAAAAGATTAAATTAAGAGAAGGGA7410.18952133713569574No Hit
GTACATGGGACAGGACACAGCTGGGTTCTGAAGCTTCTGAGTTCTGCAGC6550.1675256083992992No Hit
GGACACAGCTGGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCTCTGAG6270.1603642083455887No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT5820.1488548154021254No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTA5780.14783175825159534No Hit
GGACAGGACACAGCTGGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCT5720.14629717252580024No Hit
CAATAATACAGCACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACT5410.13836847960919216No Hit
GTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA5260.1345320152947044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.11637275087279562No Hit
GTGTTATCCATGTATTTGCAACAGCAGAGAAACAGTGACAGTGGACCATC4420.1130478151335729No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA4300.10997864368198268No Hit
GTATGGAGGAGATGTGTCTCATGGAGAAGCATCCGGGCTCAGGTGACCTT4280.10946711510671765No Hit
CTATGAGACCAGCAGCCTCTGCTCCCAGCCAGCTGTGGTATTCCAAACCA3970.10153842219010961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)