FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003670529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4392
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA200.45537340619307837No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT190.4326047358834244No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC180.4098360655737705No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG160.36429872495446264No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC140.31876138433515483No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT140.31876138433515483No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT140.31876138433515483No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA130.2959927140255009No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC130.2959927140255009No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT130.2959927140255009No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG120.273224043715847No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC120.273224043715847No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG120.273224043715847No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA110.25045537340619306No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC110.25045537340619306No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT100.22768670309653918No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT100.22768670309653918No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG100.22768670309653918No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA100.22768670309653918No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA100.22768670309653918No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC100.22768670309653918No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA100.22768670309653918No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT100.22768670309653918No Hit
CTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTCATCTCCAG80.18214936247723132No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA80.18214936247723132No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG80.18214936247723132No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT80.18214936247723132No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG80.18214936247723132No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG80.18214936247723132No Hit
TTCCTGGGCATGGCCGTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTA80.18214936247723132No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG70.15938069216757741No Hit
CTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCA70.15938069216757741No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC70.15938069216757741No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG70.15938069216757741No Hit
GTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGG70.15938069216757741No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG70.15938069216757741No Hit
GAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGG70.15938069216757741No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.15938069216757741No Hit
GGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCCGGAGGGGACC70.15938069216757741No Hit
CCTCCGGGTCAGTGGAAGCCCCAACGGGAAAGGAAACGCCCCGGGCAAAG60.1366120218579235No Hit
GGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGG60.1366120218579235No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA60.1366120218579235No Hit
CTAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGCCC60.1366120218579235No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG60.1366120218579235No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC60.1366120218579235No Hit
GAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCAGGGCT60.1366120218579235No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG60.1366120218579235No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT60.1366120218579235No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG60.1366120218579235No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC60.1366120218579235No Hit
CCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTG60.1366120218579235No Hit
GCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAAATCTGCC60.1366120218579235No Hit
CACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC60.1366120218579235No Hit
ATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAACGGGAAAG60.1366120218579235No Hit
TGTGGAGACCCATGAGCAGGGCTCCCACAATCACCACGACGATGAGGACC60.1366120218579235No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC60.1366120218579235No Hit
GTTGTGGACTTTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAG60.1366120218579235No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACAT60.1366120218579235No Hit
CAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATA60.1366120218579235No Hit
GTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGA60.1366120218579235No Hit
ATGCAGGCTCAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCC60.1366120218579235No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA60.1366120218579235No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT50.11384335154826959No Hit
GCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTT50.11384335154826959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA50.11384335154826959No Hit
GCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCC50.11384335154826959No Hit
GCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTAC50.11384335154826959No Hit
CCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCC50.11384335154826959No Hit
CACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA50.11384335154826959No Hit
GGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGT50.11384335154826959No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA50.11384335154826959No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC50.11384335154826959No Hit
TCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTG50.11384335154826959No Hit
AGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCCGGAGGGGACCCGGCC50.11384335154826959No Hit
GTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCCGGTGAGCAGG50.11384335154826959No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT50.11384335154826959No Hit
ACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATGACTACCA50.11384335154826959No Hit
GCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAA50.11384335154826959No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC50.11384335154826959No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG50.11384335154826959No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAA50.11384335154826959No Hit
CCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCG50.11384335154826959No Hit
GGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAA50.11384335154826959No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT50.11384335154826959No Hit
GATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCC50.11384335154826959No Hit
CTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGC50.11384335154826959No Hit
CACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTC50.11384335154826959No Hit
GGCCCCTGGGCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCA50.11384335154826959No Hit
TCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTG50.11384335154826959No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCT50.11384335154826959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)