FastQCFastQC Report
Tue 7 May 2024
EGAF00003670531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4582
Total Bases458.2 kbp
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC230.501964207769533No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA220.48013967699694454No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA220.48013967699694454No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC220.48013967699694454No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT200.4364906154517678No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT190.41466608467917937No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG190.41466608467917937No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA180.39284155390659103No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT170.37101702313400264No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG160.3491924923614142No Hit
CACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA150.3273679615888258No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT130.2837189000436491No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC130.2837189000436491No Hit
CTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTCATCTCCAG120.2618943692710607No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT120.2618943692710607No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG110.24006983849847227No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC110.24006983849847227No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG110.24006983849847227No Hit
CTACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAAC110.24006983849847227No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA100.2182453077258839No Hit
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA100.2182453077258839No Hit
GGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTG100.2182453077258839No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC100.2182453077258839No Hit
GGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTCATCTCCAGAACC100.2182453077258839No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC90.19642077695329552No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG90.19642077695329552No Hit
GTGGTAACCAGGTGCTCACTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGC90.19642077695329552No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT90.19642077695329552No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG80.1745962461807071No Hit
CCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAG80.1745962461807071No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA80.1745962461807071No Hit
ACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAACGG80.1745962461807071No Hit
AGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCCGGAGGGGACCCGGCC80.1745962461807071No Hit
GCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAA80.1745962461807071No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC80.1745962461807071No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA80.1745962461807071No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG80.1745962461807071No Hit
TTCCTGGGCATGGCCGTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTA80.1745962461807071No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC70.15277171540811874No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT70.15277171540811874No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA70.15277171540811874No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG70.15277171540811874No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC70.15277171540811874No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC70.15277171540811874No Hit
CTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCA70.15277171540811874No Hit
CTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATGACTACCAG70.15277171540811874No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC70.15277171540811874No Hit
GTCAGTGGAAGCCCCAACGGGAAAGGAAACGCCCCGGGCAAAGGGTCTTT60.13094718463553034No Hit
ATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAG60.13094718463553034No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA60.13094718463553034No Hit
TTTCTGGCTCATGTGGAGACCCATGAGCAGGGCTCCCACAATCACCACGA60.13094718463553034No Hit
GCTGGTAGTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCA60.13094718463553034No Hit
CCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATG60.13094718463553034No Hit
CTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTC60.13094718463553034No Hit
TGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACAC60.13094718463553034No Hit
GAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCG60.13094718463553034No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT60.13094718463553034No Hit
GGGTCAGTGGAAGCCCCAACGGGAAAGGAAACGCCCCGGGCAAAGGGTCT60.13094718463553034No Hit
TGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCC60.13094718463553034No Hit
CTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTG60.13094718463553034No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT60.13094718463553034No Hit
TCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTG60.13094718463553034No Hit
GTGGAAGCCCCAACGGGAAAGGAAACGCCCCGGGCAAAGGGTCTTTTGCA60.13094718463553034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13094718463553034No Hit
CGTTGGGGCTTCCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTA50.10912265386294195No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT50.10912265386294195No Hit
CTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCA50.10912265386294195No Hit
GCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAGCCTCA50.10912265386294195No Hit
CTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAGAGCCTCAAGA50.10912265386294195No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC50.10912265386294195No Hit
CTGCAGGCCAAGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGGG50.10912265386294195No Hit
GCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCT50.10912265386294195No Hit
GGAGAAGGTGGCAGTGGTAACCAGGTGCTCACTCAGGGCCAGGCGTTGCT50.10912265386294195No Hit
AGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCC50.10912265386294195No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG50.10912265386294195No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG50.10912265386294195No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT50.10912265386294195No Hit
GCTCAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGC50.10912265386294195No Hit
CCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTG50.10912265386294195No Hit
CTGCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCA50.10912265386294195No Hit
ATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAACGGGAAAG50.10912265386294195No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG50.10912265386294195No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG50.10912265386294195No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC50.10912265386294195No Hit
CGGCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCT50.10912265386294195No Hit
GTGGAGCCGATGGAGAAGGTGGCAGTGGTAACCAGGTGCTCACTCAGGGC50.10912265386294195No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT50.10912265386294195No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT50.10912265386294195No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA50.10912265386294195No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)