FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003670600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCCGCACAGCGACCAGGCCGCGTACGACGCGCTGGTGCGCATGGCGCA33.488372093023256No Hit
CCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCG33.488372093023256No Hit
GTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGT33.488372093023256No Hit
GCGGTGCATTCGGCGGCCCCGGTTCAGCTGGCGCCGTAGCGCGCGCGGTA22.3255813953488373No Hit
GGCCGTAGGCAGCCATGGCGCCCAGCCGGAATGGCATGGTCTTGAAGCCC22.3255813953488373No Hit
GGGAAGAATAGAGGTATCATCAAACGTGGCAAATTTTCTTTCAGGAATAA22.3255813953488373No Hit
TTTCCACACTGCAAACATTTTTTAGGGCTATCTTCTGCCCTTTGGAAGCA22.3255813953488373No Hit
CCTTAGCCCTCAAGACCGGAATATGGGCCACAGACTCCGTGTACGCCGCG22.3255813953488373No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC22.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
ACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCA22.3255813953488373No Hit
CCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCACTTATTCTAC22.3255813953488373No Hit
GCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAAC11.1627906976744187No Hit
GAGCTGCACGTTAAATGTGTGCCGCTTCCTGGAGCACATCATTGTTATCG11.1627906976744187No Hit
GGCCAGAATATGGTGAAAATCACTTAAAATATCCGTCCCTTCCATGCCTT11.1627906976744187No Hit
ATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT11.1627906976744187No Hit
CCTCAAAGCTATCCAAATATCCTCTTGCAGATTTTACAAAAAGAGTGTTT11.1627906976744187No Hit
AAGATGCTCTTCGCGTGCCTCCACCTCCCCAGTCGGTGTCCATGGCTGAG11.1627906976744187No Hit
GTGTTTATTGTTCCCAACTTATTCTCCATGTGAACCCAAGATTTAGCTCC11.1627906976744187No Hit
CTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTC11.1627906976744187No Hit
GGCTGTAATAGATCCACCACCAAGCTCATCAGAAACTTTAGCTGAGCGCT11.1627906976744187No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG11.1627906976744187No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA11.1627906976744187Clontech Universal Primer Mix Long (96% over 26bp)
CCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAG11.1627906976744187No Hit
AGTGGATACTGAGTTGATTCGCGTGGCTATCTCCATGAGATTGGATTGCA11.1627906976744187No Hit
TTCCTGAGAATGGCTTTCTCTCTCCTGATAAACTGTCTTTGCTGGAAAAA11.1627906976744187No Hit
TTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC11.1627906976744187No Hit
GAGTTTGACTGGGGCGGTACACCTGTCAAACTCCCCACCTGGCACTGTCC11.1627906976744187No Hit
CCTCTGCACATCCAGTGGTCACTACCAAGATAAAAGTGAGCCAACTGGGC11.1627906976744187No Hit
TCCAGATACTACAAAAAGAGTGTTTCAAACCTGCTCTATGAAAGGGAATG11.1627906976744187No Hit
GTTTAATAACTGGGGTGGATCTCTGTCCCTGGGACACCCATTTGGAGCCA11.1627906976744187No Hit
TTTCTCATGTATCTGCTTGCCGCCCTCTTTGGATACCTAACATTTTACGA11.1627906976744187No Hit
GAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAG11.1627906976744187No Hit
TCGCTGTTCACGACCGTCGAGCGGGTGGTCGGGTCCGGGCAGGACCCGCG11.1627906976744187No Hit
GGTTTGCTAAGGGCTGGCCCAGCAGGTGCACTCACAGGGCACTCGCTCCA11.1627906976744187No Hit
CTTATCAGGATGGTACTTCAAAGCCAGTTTCCTATAAGCCTTTTTCAATT11.1627906976744187No Hit
GGGTTGGTGACTCTGATGGCCAGTTGGGCAGCTCTTTCCACGATGGCTTT11.1627906976744187No Hit
CATTGGCAGCTGGTGATATTCGCTGCCCCACTGCTCGGAGCACTGCTGTC11.1627906976744187No Hit
ATTCCCAGTAACTTCCTTGTGTTGTGTACATTCAACTCACAGAGTTGAAC11.1627906976744187No Hit
TACCAGAAAACCGTCAAGCGCCAGATCCAAACAAACAAACAAAACCACGT11.1627906976744187No Hit
NCCAGAACGCCGCCGACCGCCTGCGCGGCGCCGTGCTCGAAACGCCCTGC11.1627906976744187No Hit
AAGAACTACTGACAACGAAGGCCGCGCCTGCCTTTCCCATCTGTCTATCT11.1627906976744187No Hit
ATCGCAGGCCGTCACGTGTAAAGCTTCTCGAGTGCCAGAGTGAACACAGA11.1627906976744187No Hit
AACACAACCTGTACAAAAAATATCCCAAATCTCATATGACTTTGAAATCT11.1627906976744187No Hit
GTGCGCAACTGCTTCACTTGGGCAACGAGCGTCGCCACGGTGTCGCGCGG11.1627906976744187No Hit
CTATATCCCGGTTCTTTCAGATGCTGCCTCTTCTATAGCCAGTAGTTATA11.1627906976744187No Hit
CCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCAAG11.1627906976744187No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.1627906976744187Clontech Universal Primer Mix Long (96% over 26bp)
GTTGCTGTGTCAGGGTATGGTGCTGGCAGATGCCTTCTACTATGTTGGCG11.1627906976744187No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT11.1627906976744187No Hit
ACCTGACATGAGCCAGCCACGCTCAGTGGCTGAACAGCATTCCCACAGCC11.1627906976744187No Hit
TCATTCACCAGGGAGGAGTCTCTGAAAATTTCGTGGGAATGTTTCTGAGG11.1627906976744187No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.1627906976744187Clontech Universal Primer Mix Long (96% over 26bp)
TGTGGCTTGCTTGTATGCATGTGGCTTGCATGTTGTGCACATGTAGCATG11.1627906976744187No Hit
GCAGAGGGGAGGGCAGGAGGAGTGAGAGGCCCAGACACTTCTTTTTTGCT11.1627906976744187No Hit
TCCCAGAACACTCAGCCCTGACACGTTAATACCAAGGGGAACAGTTAACT11.1627906976744187No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAATGCAGAGTACATG11.1627906976744187Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGTCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGA11.1627906976744187No Hit
CCCTTATAGATGTGGATACAGGTTCAAAAATTTAAGTAACTCACTTGTGA11.1627906976744187No Hit
GCTTTGTGAAAGGTAAAATCCTGTAGCCGGTGAAAGGGGTTATTCTACAA11.1627906976744187No Hit
GTTAGTGCTTAGCAAATGGGAGGCAGTTTTGTATTTAAGCATTAGCTTCA11.1627906976744187No Hit
CTTCCAGCTCCTTGACGTTGTGGACCAGGAACTTCCGGAAGCCACTGGGC11.1627906976744187No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA11.1627906976744187No Hit
GTGCATTTGCACTCTTTGCATTTGCAGGAGCCGGCGCAGGTGCAGGAGTC11.1627906976744187No Hit
GGTCTTGAACTCCTGGTCTCAAGTGATCGTCCTGCCTCAGCCTCCCAAAG11.1627906976744187No Hit
ATGTAAAAATCCTCGATAAAATACTGGCAAACCGAATCCAGCAGCGCATC11.1627906976744187No Hit
CGCGCCGCGCATCGAGGCGAAGCCCGAATGCATCTCGCCGCAGATGGCCC11.1627906976744187No Hit
GTCATCAGGACTTCCTCATTTCAGGGGGGCAGAAAAGTTCGGCGAGCGTC11.1627906976744187No Hit
GTGTGCATGGAGTGCCAGGGCCATGGGGAGCGGATCAGTCCTAAAGATAG11.1627906976744187No Hit
GGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACC11.1627906976744187No Hit
CGCGGCGCGCTGCCGATGAACATCAAGGCCCATTTCAAGAACTTCAAGGT11.1627906976744187No Hit
CTTCACCATTATTTCCAAACTGCATCAATGAATCTAAAGTGCGGGGGGAC11.1627906976744187No Hit
CACATACCTGCACATGTGCAATCAGCACATACACACTGCACATGCATGTA11.1627906976744187No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)