FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003670604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGCATTAACTCAAAAGTCCACAGTCCAACATCTCATCTGAGACAAA24.878048780487805No Hit
CCTTTGCACTCTGGTGGATCCTGAGCGGAGATCTGCAAGCTGCTTTCACC24.878048780487805No Hit
CTATTACTGAATCACAATTCATGACCTCTCAAAAGCCTGTCTCTTATACA24.878048780487805No Hit
GTACAGCCCCATGATGCCGTAGCCAGCCAGGAAGCCGAGGCTCGGTGGGC24.878048780487805No Hit
CTAGGAGACAGCCTAATGGTGGGTCTAGCTATTGACAAGAATAAGATGGT24.878048780487805No Hit
GATCCGCCGCTCCGAGGACCGCTCACCCTACATGCGCGGCATGATCAGCG24.878048780487805No Hit
GTATATAAGATCTCCATCCACAGTTAAGCTAACAAGGGTTTTTCCTGCGA12.4390243902439024No Hit
CCCTGTCGTAGGACGGCGTGTGATCAACGGGCGACCGATCCTCGTGGGCA12.4390243902439024No Hit
CCTGGAGGCAGCCGGCCAAGGCCTCTAGGGAAGAGCCCCCCCTGTCTCTT12.4390243902439024No Hit
GGCATGATCTCTGCTCACTGCACGCTCTGCCTCCCGGGTTCATGCCATTC12.4390243902439024No Hit
AGGGTGTCTCGATCGGCATCCCGCCCGCATAGGGCTCGCCGCCGCGCGTG12.4390243902439024No Hit
CGACCGTGGTCATGATGATGATCGCCATGATGGCGCTCTTCGGCACCATC12.4390243902439024No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT12.4390243902439024No Hit
ATACAGCATGGAGATGTCACCAATCGGTTTGACACAACCACCTTTTCAAC12.4390243902439024No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGCGTCAA12.4390243902439024No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA12.4390243902439024No Hit
AGATTAGCATCCACCTTTTTGCTGGCTGGCTTCCCTCGACTGACTTCTCT12.4390243902439024No Hit
GTCATAGGGCGTGTCCTATCTTAATACGGATGAACAGGAGGGATGCCCCT12.4390243902439024No Hit
GTTTTAACCTTGCCAAGCAGGCAGTTACTCTCAGGAAATGAAAGATACAA12.4390243902439024No Hit
AGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCG12.4390243902439024No Hit
CCAAAGTGCTGGGATTACAGGCATGAGCCACTGCGTCCAACTGACAATTA12.4390243902439024No Hit
GAACATGCTCCTTTAGCTCGGAGAAGTTTGTTATTACTGACCTTTTGAAG12.4390243902439024No Hit
GAGCAAAGGACTAATTTGCTAAAGTGAACTGTAGGCTCTGTCTCTTATAC12.4390243902439024No Hit
GATAAGGAAATGGAGGCTCAGAGGGGTTGAGTCATTTGTCTTGGGTCACA12.4390243902439024No Hit
ATGTTGTTGGACACCAGTTCCAGCACCAGGATCAGCGCGGCCGTCATCAG12.4390243902439024No Hit
TCTCAGCTCACTGCAAGCTCTGCCTCCCAGGTTCATGCCATTCTCCCGCC12.4390243902439024No Hit
GCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGG12.4390243902439024No Hit
GAAATTTCTGTACTTTCCACTCACTTTTTCTGAAACCTGAAACTGCTCTA12.4390243902439024No Hit
TCCGAACGGTATAGGGTTAGCGCCTTACCTGCGAATGAAGCAGGAAGGAA12.4390243902439024No Hit
CTCCTGGGAGGTGAGCCAAGTGGACGAGATGCTGGCCCGGATGGAGCGGC12.4390243902439024No Hit
GCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGA12.4390243902439024No Hit
CTGCAGGCCCTTGTGCGTTTGCACAATGTTTGTGTGCTGGAAACTGAAAA12.4390243902439024No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.4390243902439024Clontech Universal Primer Mix Long (95% over 21bp)
GCATCCATGCCAACGGGCGCGGCAGGCTGGACGAGATCGCGCGGCGCATG12.4390243902439024No Hit
CCAAGGAAGGATGGGACACTTGGTCTCCAGGAGCCACAGACGATATGATT12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)