FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003670608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAACTGTCAACTGGAGAGATTCCGTCAACGATCCGCCAGCTCAAGAT212.5No Hit
ATCGGAAGAGCGTCGTGTAGGGAAGAGGTATCTGGTGCTATCCCATGTAC212.5Illumina Single End PCR Primer 1 (95% over 24bp)
GTTTCCATGTCCAGCGCGACAGCCCACGCGTAAGCGCCATTCGCCAAACC212.5No Hit
GAATTATCTCCGTGGTTCATTTGATGATCCTTCATCTCAGGATTATTTTG212.5No Hit
CTAGAATTCCCCATTACTATGGCTTCTGCTGACTTCTGATCGTTCAGCGG16.25No Hit
GTATTAATACTATGTTGGATAATTTTGGAAATGAGAACGAAATCGTTGTA16.25No Hit
ACCTCGACCACTGTCACAACGACGTGCGGCAGGCCCCTTTGCTGCGCGAC16.25No Hit
GGGACACCCGCGCCCTCCAGCAGCCGGCACCCACGACGGACGCACCTCCG16.25No Hit
CGGTTCTGAGAAGAAACAAGCCATCAAGGAAGCCCTGAAGCCCCAGGCAC16.25No Hit
CCTTCAGGGTGCTGTGACTTAGCCTTCCGTCCAGACTGGCTATGTTCCAG16.25No Hit
GGGCGTGCAATGCGCCTGCGACGCTGGCCAGGAGGTCCAGGGCCAGATGT16.25No Hit
TTTTTAGGTAATGAGGAAGATTCTCGCTTCCATTTATCAGAGGAATCTAA16.25No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)