FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003670611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT27.6923076923076925No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC27.6923076923076925Clontech SMART CDS Primer II A (96% over 26bp)
GGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG13.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
GACCTTCACCGTGCCCGCTGCAGATCTGGCGCGCTCGATGGACCTGCTCG13.8461538461538463No Hit
CGACCACCGAGATCTACACTTTTTTTTTCGTCGGCAGCGCTGTCTCTTAT13.8461538461538463Illumina Single End PCR Primer 1 (95% over 24bp)
GCCTCGACGTGTGGGTAGTGCCCGAGGTCGGGCCACGTCTCCTTTCGCGC13.8461538461538463No Hit
GTCCGGTTGCCGCGTCGGGAGATCGCAGTCCAACTATTGCGCAATGAACA13.8461538461538463No Hit
CTCATACACAGACGCTGCTGGAACAAGGAGTCGGGTCGTGCCCGCGTTCG13.8461538461538463No Hit
CCGCCGCCCTGAGCCGCGCCAACAAGGATCTGCGCGCGGAGAACAACCAG13.8461538461538463No Hit
GCATGGCCCCACTTGCGCTCCGCATCCTGGGCGATGAACTTGGCCAGTTC13.8461538461538463No Hit
GCCGACCACCGGCCCGAGCGCAAGCAGGCATGGCGGCCTGCATTGCAGAA13.8461538461538463No Hit
GGCGTGCTCCGCTCAGGCTGCCGCGCGGGCACGGGCCTGCAGGCGCAGGC13.8461538461538463No Hit
ATCCGGAGATCGACGAGACGCGACGTGTGCAACGGCGGCCGACGACGACC13.8461538461538463No Hit
GGGGAGAGAACGCCTGCCGAGACGAGCAACGTTGCAGTTGCCACGAGCGG13.8461538461538463No Hit
CATCAACAAACTAAACCAAAACCACGCTAAACAGATAACATCAAGCAAGT13.8461538461538463No Hit
GTATCAACGCAGAGTACATGGGGAATGATACGGCGACCCTGTCTCTTATA13.8461538461538463No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG13.8461538461538463Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCGTGTTCCGCAGCGTGCTGGAAGACCTCATGCCGCTTGCCGACGCCA13.8461538461538463No Hit
CACTGCATTCTCCACCTCCCGGGTTCAAGCGATTCTCCTGCCTCAGCCTC13.8461538461538463No Hit
GAAATAACAAATAGTTCGCTCATGACTATTTTTTATCAACCCAAAGGTAC13.8461538461538463No Hit
TCGGAGGACCGCGTGACCGTGTCGCCCGACCCGAAGGGCGGACGAGCGCG13.8461538461538463No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG13.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGTGGCGACGGCAGCGAGGCCGAAAAAAAAA13.8461538461538463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA13.8461538461538463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)