FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003670612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27.6923076923076925Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCCTGTCTCTTAT27.6923076923076925No Hit
CCTGTAATCTCAGTTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACC13.8461538461538463No Hit
GCGCATGCCGTCGGCGTCGCGATCCGGCGCCGAGGCGGCGAACAGGCGCG13.8461538461538463No Hit
GTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGTACGGCGA13.8461538461538463Clontech Universal Primer Mix Long (96% over 27bp)
GTCCACTACGTCAACCCGCCCAAGATCTTCTCGGGCTGCGAAGGCAGCTA13.8461538461538463No Hit
GAGTTCAGACGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAA13.8461538461538463Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA13.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
GATCGGGGCCGAGTGCGGCGCCGACCTCTTGTTGGAAGCGGGATCTGGCT13.8461538461538463No Hit
CCACGTCGCGGACCTTGACTCGCGCTCGTCCGCCCTTCGGGTCGGGCGAC13.8461538461538463No Hit
ACGATGCAGTCGGTGTAACCACCAAAGCGCGGCATGCCGAACACGCGCTC13.8461538461538463No Hit
GCTCTTCATGCCGACGCCGACCACCGAAATCTTGGCGACATTGGTGTCGG13.8461538461538463No Hit
GGCTTGTTCAGGGCGATAAGTACCAAATCTTCGGCTTCCCGAGGTTCAAT13.8461538461538463No Hit
GTACTTTTTTTTTTTTCGGCCTCGCTGCCGTCGCCACCCATGTACTCTGC13.8461538461538463No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT13.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
CGCTGCGGGCCGATCAGCCGGCTCAGCGGGCCGCGATAGCACGGGCGCTC13.8461538461538463No Hit
TGAGAGGCGTACGCGGCTGCGGTGCGAACGCCGCGACGAGCGCATAGCCG13.8461538461538463No Hit
GATCCTGTCCACCCACATCGTCGAAGACGTGACCGACCTGTGCCCCACCA13.8461538461538463No Hit
ACACCGAGGAACGCTGGCGCTTCGAGCGCAGGTGGATCGGCGCGCTCGAG13.8461538461538463No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA13.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTCCTGCATCTGTCGGTTGTGCGCTTCCTCGGTCACGCTGGGTCCG13.8461538461538463No Hit
GGTCGCCTCATTGCCGAGCACACGATAGAACGGGTCGAACACGCGCGCGC13.8461538461538463No Hit
GGTCGCCGTATCATTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATA13.8461538461538463No Hit
CGCTGCCGACGAAAAAAAAAGTGTAGATCTCGGTGGTCGCTGTCTCTTAT13.8461538461538463Illumina Single End PCR Primer 1 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)