FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003670613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGATATTTGCAATGAATGTCTGTTGCACTTGAGCATGCAAATGGACT27.4074074074074066No Hit
CGCATGTCCAGGGTGGTCTGCAACGACACCTCGAAGGTGCGCAGATTGCG13.7037037037037033No Hit
ACGTGGTGGAAAAGCTCGGCACGCCCATCGGTGCCTTGAGTGCCGACTTC13.7037037037037033No Hit
GGACGAAACTATTGAACAGAAGGGCATTCATGGCTGAAGGGGAAGATCTG13.7037037037037033No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTT13.7037037037037033No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTGTTTAGAGATCGGAAGAGCGTCGTGTAGGGACTGTCTCTTATACA13.7037037037037033Illumina Single End PCR Primer 1 (96% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC13.7037037037037033Clontech SMART CDS Primer II A (96% over 26bp)
CACGACGCTCTTCCGATCTCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAA13.7037037037037033No Hit
GTCGGAGGTGGTCTGTTCGGCGAGCCGCATCAGGCGCTTCAGCCCGCGCT13.7037037037037033No Hit
GACGTGAACGCACTTGTCGCGCCTGAAGGTCGTGGCAACGATGGTGCGCA13.7037037037037033No Hit
CTCTCTGGTCTACTTGAAGTAACTCCGTCATTCTACGAGAATGACGGAGT13.7037037037037033No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
CTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGA13.7037037037037033No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA13.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
ACGTGCGCGTGCGCCGAGCGATCTCCGCGGCCATCGACCGCAAGGCAGTG13.7037037037037033No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA13.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
CATAAACCAAACAATATAAAACAATTCACTAAAACAAAAACAAAACAAAA13.7037037037037033No Hit
ATTCTTAAAGCACCTTGACCGAATATAACACTTTGCTCCGTATAATCCGA13.7037037037037033No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG13.7037037037037033No Hit
CTCCCTACACGACGCTCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13.7037037037037033Illumina Single End PCR Primer 1 (95% over 21bp)
CTTCGTCGCTGACGATCATCGTTCCCGGGATCGTGTCTGCGGCCGGGGCA13.7037037037037033No Hit
GACCCGGCCGAGCTGATGGGCCCGATGACCTTCGACCAGGACTACGCGCA13.7037037037037033No Hit
GGTGGTGACCAACGTGGTCGAGAACGCCGTCCGGCACGGCGAGGGCGTCG13.7037037037037033No Hit
GCTCCTCAGATGAACTTCTGCTGGGCCAGGAACTCGGCGAGCTTCGTGCC13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)