FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003670614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003670614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAGGAAACTGAGCAACCCTTCAAAGCGATGGGCATAGGCTCCGCCTA27.4074074074074066No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACCCAATT13.7037037037037033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13.7037037037037033No Hit
CTGAACTCCTGGTGGGGCTTCATCCTCGCCGGCCTGCTCGCCGTTCCGCT13.7037037037037033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13.7037037037037033No Hit
TCCCTACACGACGCTCTTCCGATCTCTAAACAGGATCTGTCTCTTATACA13.7037037037037033Illumina Single End PCR Primer 1 (100% over 25bp)
CGACGAGTCCCTGCGTTCTGAACTGCGTAAAGCTGGCTTCGTTACTCGTG13.7037037037037033No Hit
GAAGTCGGCACTCAAGGCACCGATGGGCGTGCCGAGCTTTTCCACCACGT13.7037037037037033No Hit
GTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGA13.7037037037037033No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACCTGTCTCTT13.7037037037037033Clontech SMART CDS Primer II A (95% over 21bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC13.7037037037037033Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG13.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA13.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
GCCGAGGTGACCGCGCGGCCGCCGAAGCAGGCCGGCCAGATCGTCAAGGA13.7037037037037033No Hit
CCTCGTTGAGGTCCTCCCGGCGAGGAGTACCGGACGTGCACGTGCCATGC13.7037037037037033No Hit
GCCCACTTCACCGTCAGGATGTCCGCCGCGATCTTCGCGGCGTTGACGCC13.7037037037037033No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
GGTCACGACGCCCTCGCCGTGCCGGACGGCGTTCTCGACCACGTTGGTCA13.7037037037037033No Hit
GACGCGGGTCGAGCGACTACGGACGAGCACCGGGCCAGCCGGAGCAGCCG13.7037037037037033No Hit
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG13.7037037037037033Illumina Single End PCR Primer 1 (100% over 22bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
GTGCAGGTGCCGGTGCCGCTGGTGGGCATCAACAACCGCAATCTGCGCAC13.7037037037037033No Hit
GCGCCCATTCGACGTTCTGGATCTTCGCGACGATGCCCACCATGGCGAGC13.7037037037037033No Hit
ATACCACTTGGCGGCGAGTAGGTATTCAGATGGATGAGATTTTAATTGTC13.7037037037037033No Hit
GCCGGGTCACCACCCTGGGGCGCGGCGGATCGGACACCTCGGCGGTGGCC13.7037037037037033No Hit
GAAGTGGTATCATGCCGCGCGATTGTATTTAAAAGATCGTCTGAAGCTGG13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)